Germ Cell Degeneration in High-Temperature Treated Pufferfish, Takifugu rubripes
Germ Cell Degeneration in High-Temperature Treated Pufferfish, Takifugu rubripes
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DOI：
10.1159/000228723
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发表时间：
2009-01-01
影响因子：
2.3
通讯作者：
Matsuyama, M.
中科院分区：
文献类型：
--
作者：
Lee, K. H.;Yamaguchi, A.;Matsuyama, M.
在许多脊椎动物性别分化的关键敏感期，温度、社会行为和盐度等外源因素起着至关重要的作用。在鱼类、两栖动物和爬行动物中，温度处理已知会诱导全雄性(或雌性)个体，并且已经研究了与性别分化相关的基因。日本河豚拥有脊椎动物中最紧凑的基因组，在比较基因组分析方面具有巨大的研究潜力。在本研究中，我们在分子水平上描述了温度处理的河豚性腺形态和VasA(生殖细胞标记)和dmrt1(支持细胞标记)的表达与发育的关系。为了研究温度与性腺发育的关系，在性腺发育的早期，河豚被暴露在高温(32摄氏度)条件下。形态观察表明，高温处理不影响卵巢腔特征所确定的性分化；但高温处理会导致性腺退化，性腺退化，没有生殖细胞。RT-PCR结果显示，在生殖细胞变性的性腺中没有VasA的表达。原位杂交结果表明，dmrt1在生殖细胞变性卵巢的体细胞中有表达。这些结果表明，在性腺发育早期进行高温处理会导致河豚生殖细胞退化和卵巢体细胞雄性化。版权所有(C)2009 S.Karger AG，巴塞尔
Exogenous factors such as temperature, social behavior, and salinity play a crucial role during the critical sensitive period of sex differentiation in many vertebrates. In fishes, amphibians, and reptiles temperature treatment is known to induce all-male (or female) individuals, and genes related to sex differentiation have been studied. The Japanese pufferfish, Takifugu rubripes, possesses the most compact genome among vertebrates and has immense potential for studies focusing on comparative genome analysis. In this study, we describe gonadal morphology and vasa (germ cell marker) and dmrt1 (Sertoli cell marker) expression on a molecular level in relation to the development of temperature-treated pufferfish. To investigate the relationship between temperature and gonadal development, pufferfish were exposed to high-temperature conditions (32 degrees C) during early gonadal development. Morphological observations showed that this high-temperature treatment did not influence sexual differentiation as determined by ovarian cavity characteristics; however, high-temperature treatment induces gonadal degeneration that is devoid of germ cells. RT-PCR results revealed no vasa expression within germ cell-degenerated gonads. In situ hybridization results showed that dmrt1 was expressed in somatic cells of germ cell-degenerated ovaries. These results suggest that high-temperature treatment during early gonadal development induces germ cell degeneration and masculinization of ovarian somatic cells in pufferfish. Copyright (C) 2009 S. Karger AG, Basel