Preparation of Adult Pig Pancreatic Cells: Comparative Study of Methods with or without Proteolytic Enzymes
Preparation of Adult Pig Pancreatic Cells: Comparative Study of Methods with or without Proteolytic Enzymes
复制标题
成年猪胰腺细胞的制备：有或无蛋白水解酶方法的比较研究
DOI：
10.1177/096368979400300409
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发表时间：
1994
影响因子：
3.3
通讯作者：
Y. Hashimoto
中科院分区：
文献类型：
--
作者：
H. Ohgawara;A. Kobayashi;Suk;T. Akaike;Y. Hashimoto
描述了用酶或自动消化法制备成年猪胰腺细胞的方法，并对这些方法进行了比较。通过胰腺自动消化制备的分散胰腺细胞获得了更多的细胞，它们具有最高的活力和胰岛素分泌反应，用双硫腙染色成红色。另一方面，当胰腺暴露于胶原酶或edta - disase时，观察到许多受损细胞，只有少数细胞被双硫腙染红。在使用自动消化程序制备的细胞中，观察到胰岛素分泌对葡萄糖刺激的反应长达6小时，并观察到胰岛素分泌对葡萄糖的反应增加约2至3倍。通过胶原酶或edta - disase暴露获得的细胞对低（5.5 mmol/l）或高（16.7 mmol/l）葡萄糖浓度均无分泌反应。本报告表明，从成年猪胰腺中制备胰腺内分泌细胞是可能的，这是一种获得胰腺细胞移植或研究其生化或生理特性的廉价方法。
Methods for the preparation of adult pig pancreatic cells using enzymatic- or auto-digestion are described, and a comparison of these methods was performed. A greater number of cells staining red with dithizone with the highest viability and insulin secretory response to glucose stimulation were obtained with dispersed pancreatic cells prepared by auto-digestion of the pancreas. On the other hand, many damaged cells were observed when the pancreas was exposed to collagenase or EDTA-Dispase and only a few cells stained red with dithizone. Insulin secretion in response to glucose stimulation was observed for up to 6 h in cells prepared using the auto-digestion procedure and an approximately 2-to 3-fold increase in insulin secretion was seen in response to glucose. Cells obtained by the collagenase or EDTA-Dispase exposure did not show a secretory response to either low (5.5 mmol/l) or high (16.7 mmol/l) glucose concentrations. This report shows that it is possible to prepare pancreatic endocrine cells from the adult pig pancreas which represents an inexpensive way to obtain cells for pancreatic cell transplantation or studies of their biochemical or physiological properties.