CLONING, EXPRESSION, AND GENE STRUCTURE OF A G-PROTEIN-COUPLED GLUTAMATE RECEPTOR FROM RAT-BRAIN
CLONING, EXPRESSION, AND GENE STRUCTURE OF A G-PROTEIN-COUPLED GLUTAMATE RECEPTOR FROM RAT-BRAIN
复制标题
大鼠脑 G 蛋白偶联谷氨酸受体的克隆、表达和基因结构
DOI：
10.1126/science.1656524
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发表时间：
1991-05-31
期刊：
影响因子：
56.9
通讯作者：
HAGEN, FS
中科院分区：
文献类型：
--
作者：
HOUAMED, KM;KUIJPER, JL;HAGEN, FS
通过在非洲爪蟾卵母细胞中的功能表达，克隆了编码来自大鼠脑的G蛋白偶联谷氨酸受体Glu（G）R的互补DNA。互补DNA编码一个1199个氨基酸的蛋白质，含有一个七跨膜基序，两侧是大的氨基和羧基末端结构域。这种受体缺乏任何氨基酸序列相似性与其他G蛋白偶联受体，这表明它可能是一个新的亚家族的成员。两个内含子侧翼的中央核心的存在表明，Glu（G）R可能已经通过外显子改组进化。Glu（G）R在卵母细胞中的表达受使君子酸>谷氨酸>鹅膏蕈氨酸>反式-1-氨基环戊基-1，3-二羧酸的激活，受2-氨基-3-膦酰基丙酸的抑制。激活被波氏百日咳毒素阻断。这些特性是某些代谢型谷氨酸受体的典型特性。
A complementary DNA encoding a G protein-coupled glutamate receptor from rat brain, Glu(G)R, was cloned by functional expression in Xenopus oocytes. The complementary DNA encodes a protein of 1199 amino acids containing a seven-transmembrane motif, flanked by large amino- and carboxyl-terminal domains. This receptor lacks any amino acid sequence similarity with other G protein-coupled receptors, suggesting that it may be a member of a new subfamily. The presence of two introns flanking the central core suggests that Glu(G)R may have evolved by exon shuffling. Expressed in oocytes, Glu(G)R is activated by quisqualate > glutamate > ibotenate > trans-1-aminocyclopentyl-1,3-dicarboxylate, and it is inhibited by 2-amino-3-phosphonopropionate. Activation is blocked by Bordella pertussis toxin. These properties are typical of some metabotropic glutamate receptors.