Culture, isolation and propagation of Babesia caballi from naturally infected horses
Culture, isolation and propagation of Babesia caballi from naturally infected horses
复制标题
自然感染马中卡巴贝斯虫的培养、分离和繁殖
作者：
E. Zweygarth;L. Lopez;J. Nurton;A. Guthrie
抽象的。来自南非的13匹马的血液样本，其中5匹为卡巴里马血清阳性，8匹为两种B。caballi和马泰勒虫进行体外培养以鉴定携带者动物。在培养开始前，所有动物的Giemsa染色血涂片均未检出寄生虫血症。培养物在富含L-半胱氨酸的培养基中开始，在氧气减少的气体混合物中或在5%CO2-空气气氛中。所有5只动物均为B血清阳性。caballi被鉴定为使用氧气减少的气氛的携带者动物，而在正常的气氛条件下只有四个样品成为培养阳性。在8个样本中，两种B均为血清阳性。caballi和T.马，两个被确定为两者的携带者。其余6个样本被确定为仅携带T。等。
Abstract. Thirteen blood samples of horses from South Africa, five of which were seropositive for Babesia caballi and eight for both B. caballi and Theileria equi, were subjected to in vitro culture to identify carrier animals. None of the animals had a detectable parasitaemia on Giemsa-stained blood smears before culture initiation. Cultures were initiated in L-cysteine-enriched medium, either in an oxygen-reduced gas mixture or in a 5% CO2-in-air atmosphere. All five animals seropositive for B. caballi were identified as carrier animals using an oxygen-reduced atmosphere, whereas only four samples became culture positive under normal atmospheric conditions. Among the eight samples seropositive for both B. caballi and T. equi, two were identified as carriers for both. The remaining six samples were identified as carrying only T. equi.