Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin.
Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin.
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DOI：
10.1093/clinchem/37.9.1618
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发表时间：
1991-09
影响因子：
9.3
通讯作者：
H. Lilja;A. Christensson;Ulrika Dahlén;M. Matikainen;O. Nilsson;K. Pettersson;T. Lövgren
中科院分区：
文献类型：
--
作者：
H. Lilja;A. Christensson;Ulrika Dahlén;M. Matikainen;O. Nilsson;K. Pettersson;T. Lövgren
前列腺特异性抗原（PSA），一种丰富的前列腺分泌的丝氨酸蛋白酶，血清浓度的免疫测量，经常用于监测前列腺癌患者，虽然它还没有被确定是否这种免疫反应代表PSA酶原，活性蛋白酶，或PSA复合细胞外蛋白酶抑制剂。为了表征血清中PSA的免疫反应性，我们在三种非竞争性PSA测定法的设计中使用产生的抗PSA的单克隆抗体和抗α 1-抗胰凝乳蛋白酶的多克隆兔IgG：测定法T，其检测作为活性蛋白酶存在时和与α 1-抗胰凝乳蛋白酶复合时的PSA;测定法F，其识别活性蛋白酶但最难检测与α 1-抗胰凝乳蛋白酶复合的PSA;和测定C，其对与α 1-抗胰凝乳蛋白酶复合的PSA具有特异性。我们用这三种方法测定了64例患者血清和4例患者血清凝胶层析后流出液中的PSA免疫反应性。这确定了PSA和α 1-抗胰凝乳蛋白酶之间的80- 90-kDa复合物是血浆中PSA免疫反应性的主要部分;免疫反应性25- 40-kDa化合物是次要部分。
Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.