COMPARISON OF RAPESEED CULTIVARS AND RESYNTHESIZED LINES BASED ON ALLOZYME AND RFLP MARKERS
COMPARISON OF RAPESEED CULTIVARS AND RESYNTHESIZED LINES BASED ON ALLOZYME AND RFLP MARKERS
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DOI：
10.1007/bf00220859
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发表时间：
1995-07-01
影响因子：
5.4
通讯作者：
KARLSSON, B
中科院分区：
文献类型：
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作者：
BECKER, HC;ENGQVIST, GM;KARLSSON, B
经常有人建议使用从B再合成油菜籽（甘蓝型油菜）。campestris和B.扩大其遗传基础。本研究的目的是双重的：（1）比较内的再合成油菜的遗传变异与世界范围内收集的油菜品种，（2）比较遗传距离估计从RFLP标记和距离估计从相对较少的等位酶标记。对17个人工合成材料和24个油菜品种进行了调查。遗传距离估计的基础上电泳的7个等位酶，共38个不同的带，或基于RFLP数据的51探针/酶组合，共355个不同的带。等位酶分析和RFLP分析结果基本一致。遗传距离，估计从两个独立的组的RFLP数据与25和26探针/酶组合，分别是高度相关的，因此约50个RFLP标记是足够的表征油菜籽材料具有很大的遗传多样性。这些品种被聚为三个组：（1）欧洲和北方美洲起源的春油菜籽，（2）欧洲和北方美洲起源的冬油菜籽，和（3）亚洲起源的油菜籽。一些再合成的油菜品系与欧洲冬油菜品种相似，而另一些则具有相当独特的模式。因此，人工合成油菜是扩大现有甘蓝型油菜育种材料遗传变异的一个有价值的来源。然而，不同的线路在其适用性方面存在很大差异。
It has frequently been suggested to use the resynthesis of rapeseed (Brassica napus) from B. campestris and B. oleracea to broaden its genetic base. The objective of the present study is twofold: (1) to compare the genetic variation within resynthesized rapeseed with a world-wide collection of oilseed rape cultivars, and (2) to compare genetic distances estimated from RFLP markers with distances estimated from a relatively small number of allozyme markers. We investigated 17 resynthesized lines and 24 rapeseed cultivars. Genetic distances were estimated either based on the electrophoresis of seven allozymes, with a total of 38 different bands, or based on RFLP data of 51 probe/enzyme combinations, with a total of 355 different bands. The results of allozyme and RFLP analyses agreed reasonably well. Genetic distances, estimated from two independent sets of RFLP data with 25 and 26 probe/enzyme combinations respectively, were highly correlated; hence about 50 RFLP markers are sufficient to characterize rapeseed material with a large genetic diversity. The cultivars were clustered into three groups: (1) spring rapeseed of European and Northern American origin, (2) winter rapeseed of European and Northern American origin, and (3) rapeseed of Asian origin. Several of the resynthesized rapeseed lines were similar to European winter rapeseed cultivars, whereas others had quite unique patterns. It is concluded, that resynthesized rapeseed is a valuable source for broadening the genetic variation in present breeding material of Brassica napus. However, different lines differ widely in their suitability for this purpose.