Adenovirus core protein V interacts with p32 - a protein which is associated with both the mitochondria and the nucleus
Adenovirus core protein V interacts with p32 - a protein which is associated with both the mitochondria and the nucleus
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DOI：
10.1099/0022-1317-79-7-1677
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发表时间：
1998-07-01
影响因子：
3.8
通讯作者：
Russell, WC
中科院分区：
文献类型：
--
作者：
Matthews, DA;Russell, WC
腺病毒蛋白V与含有DNA的病毒核心结合，并作为衣壳和核心之间的桥梁发挥作用。使用蛋白V作为“诱饵”，用人cDNA文库进行酵母双杂交分析，选择细胞蛋白，p32 -先前描述为与剪接因子ASF/SF 2相关。通过p32的表达和纯化以及抗体的制备，我们通过包括免疫沉淀在内的多种方法证实了p32与V的结合。我们证明，p32主要位于细胞质中与线粒体，但也可以在细胞核中检测到不同的颗粒和小管。通过使用共聚焦显微镜和免疫荧光检查感染的细胞，我们能够遵循蛋白V和p32的细胞内位置，并推测p32是将蛋白质导入细胞核的系统的一部分，腺病毒劫持该过程以将其基因组递送到细胞核。
Adenovirus protein V is associated with the DNA-containing virus core and functions as a bridge between the capsid and the core. A yeast two-hybrid analysis performed with a human cDNA library using protein V as 'bait' selected a cellular protein, p32 - described previously as associated with the splicing factor ASF/SF2. By expression and purification of p32 and preparation of an antibody we confirmed the binding of p32 to V by a variety of methods including immune precipitation. We demonstrated that p32 was primarily located in the cytoplasm in association with mitochondria but could also be detected in the nucleus as distinct granules and tubules. By examining infected cells using confocal microscopy and immunofluorescence we were able to follow the intracellular locations of protein V and p32 and it is postulated that p32 is part of a system which imports proteins to the nucleus and that adenovirus hijacks this process to deliver its genome to the nucleus.