Quantitative trait loci controlling light and hormone response in two accessions of Arabidopsis thaliana.
Quantitative trait loci controlling light and hormone response in two accessions of Arabidopsis thaliana.
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发表时间：
2002-02
期刊：
影响因子：
3.3
通讯作者：
J. Borevitz;J. Maloof;J. Lutes;T. Dabi;Joanna L. Redfern;Gabriel T. Trainer;Jonathan D. Werner;T. Asami;C. Berry;D. Weigel;J. Chory
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作者：
J. Borevitz;J. Maloof;J. Lutes;T. Dabi;Joanna L. Redfern;Gabriel T. Trainer;Jonathan D. Werner;T. Asami;C. Berry;D. Weigel;J. Chory
我们使用重组自交系 (RIL) 绘制了拟南芥佛得角群岛 (Cvi) 和兰兹伯格直立 (Ler) 种质之间负责光和激素反应自然变异的数量性状位点 (QTL)。在四种光环境下测量下胚轴长度：白光、蓝光、红光、远红光和黑暗。此外，还使用白光加赤霉素（GA）和暗光加油菜素类固醇生物合成抑制剂油菜素唑（BRZ）来检测激素效应。鉴定出 12 个 QTL，这些 QTL 映射到先前未知的影响光响应的基因座，以及已从已知突变中鉴定出候选基因的基因座。一些 QTL 在所有环境中都起作用，而另一些则表现出基因型与环境的相互作用。建立了一个全局阈值来识别两个基因座之间的显着上位相互作用，而这两个基因座本身几乎没有主要影响。 LIGHT1 是一个主要 QTL，已在近等基因系 (NIL) 中得到证实，并定位到在所有光环境下都有作用的新基因座。 electeta 突变可以解释 HYP2 QTL 在蓝色、BRZ 和黑暗环境中的效应，但不能解释远红环境中的效应。 LIGHT2 也在 NIL 中得到证实，它在白光和红光下具有作用，并显示出与 GA 的相互作用。 LIGHT2 的表型和图谱位置表明光感受器 PHYB 作为候选基因。因此，光和激素反应的自然变化定义了控制野生种的光反应的新基因和已知基因。
We have mapped quantitative trait loci (QTL) responsible for natural variation in light and hormone response between the Cape Verde Islands (Cvi) and Landsberg erecta (Ler) accessions of Arabidopsis thaliana using recombinant inbred lines (RILs). Hypocotyl length was measured in four light environments: white, blue, red, and far-red light and in the dark. In addition, white light plus gibberellin (GA) and dark plus the brassinosteroid biosynthesis inhibitor brassinazole (BRZ) were used to detect hormone effects. Twelve QTL were identified that map to loci not previously known to affect light response, as well as loci where candidate genes have been identified from known mutations. Some QTL act in all environments while others show genotype-by-environment interaction. A global threshold was established to identify a significant epistatic interaction between two loci that have few main effects of their own. LIGHT1, a major QTL, has been confirmed in a near isogenic line (NIL) and maps to a new locus with effects in all light environments. The erecta mutation can explain the effect of the HYP2 QTL in the blue, BRZ, and dark environments, but not in far-red. LIGHT2, also confirmed in an NIL, has effects in white and red light and shows interaction with GA. The phenotype and map position of LIGHT2 suggest the photoreceptor PHYB as a candidate gene. Natural variation in light and hormone response thus defines both new genes and known genes that control light response in wild accessions.