Structural Basis of the Association of HIV-1 Matrix Protein with DNA
Structural Basis of the Association of HIV-1 Matrix Protein with DNA
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DOI：
10.1371/journal.pone.0015675
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发表时间：
2010-12-23
期刊：
影响因子：
3.7
通讯作者：
Clore, G. Marius
中科院分区：
文献类型：
--
作者：
Cai, Mengli;Huang, Ying;Clore, G. Marius
HIV-1 基质 (MA) 是一种多功能蛋白质，合成为多蛋白，在病毒成熟过程中被蛋白酶切割。 MA 含有一组碱性残基，其作用存在争议。提议的功能包括膜锚定、促进病毒组装和指导病毒 DNA 的核输入。由于 MA 已被报道为预整合复合物 (PIC) 的组成部分，因此我们使用 NMR 来探测其与其他 PIC 成分的相互作用。我们证明 MA 与 DNA 相互作用，这可能足以解释它与 PIC 的关联。
HIV-1 matrix (MA) is a multifunctional protein that is synthesized as a polyprotein that is cleaved by protease during viral maturation. MA contains a cluster of basic residues whose role is controversial. Proposed functions include membrane anchoring, facilitating viral assembly, and directing nuclear import of the viral DNA. Since MA has been reported to be a component of the preintegration complex (PIC), we have used NMR to probe its interaction with other PIC components. We show that MA interacts with DNA and this is likely sufficient to account for its association with the PIC.