Sensitive Detection of Spores Using Volume-Amplified Magnetic Nanobeads

Sensitive Detection of Spores Using Volume-Amplified Magnetic Nanobeads
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DOI:
10.1002/smll.201102632
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发表时间:
2012-07-23
期刊:
影响因子:
13.3
通讯作者:
Nilsson, Mats
Nilsson, Mats
中科院分区:
材料科学1区
文献类型:
--
作者:
de la Torre, Teresa Zardan Gomez;Ke, Rongqin;Nilsson, Mats

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提出了一种基于磁性纳米珠的无底物方法,用于以免疫分析形式灵敏检测孢子。该方法可检测球芽孢杆菌孢子(炭疽杆菌的非致病性模拟物),检测限为 50 个孢子,反应时间为 135 分钟。该研究展示了磁性纳米珠在检测 DNA 以外的生物分子方面的多功能性。
A magnetic-nanobead-based, substrate-free method for the sensitive detection of spores in an immunoassay format is presented. The method is shown to detect Bacillus globigii spores, the non-pathogenic simulant of Bacillus anthracis, with a limit-of-detection of 50 spores with a reaction time of 135 min. The study shows the versatility of magnetic nanobeads for detection of biological molecules other than DNA.