Comparative genome mapping: mouse and rat homologies revealed by fluorescence in situ hybridization.
Comparative genome mapping: mouse and rat homologies revealed by fluorescence in situ hybridization.
复制标题
比较基因组作图：通过荧光原位杂交揭示小鼠和大鼠的同源性。
DOI：
10.1006/geno.1997.5090
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hozier,JC
中科院分区：
文献类型：
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作者：
Scalzi,JM;Hozier,JC
小鼠和大鼠基因组研究对于将啮齿动物用作生物学和人类遗传病的模型至关重要。在这项研究中，比较细胞遗传学图谱的个体同源的小鼠(小鼠)和大鼠(褐家鼠)的染色体区域被定义为跨物种的荧光原位杂交。这种“Zoo-FISH”方法可以直观地观察一个物种的DNA片段在进化分化物种的有丝分裂染色体上的位置。将显微切割产生的小鼠全染色体涂片(WCP)探针与简并寡核苷酸扩增(DOP)聚合酶链式反应(DOP)探针在含有小鼠(参照种)和大鼠(分歧种/比较种)中期染色体混合物的玻片上进行杂交。使用6个不同的小鼠WCP，7个大鼠染色体上的8个区域被证明在进化上在这些物种之间是保守的。小鼠(MMU)和大鼠(RNO)基因组同源性的特异染色体位置包括：MMU 1至RNO 9q21-Q36和RNO 13从Q11带到端粒，MMU 4到RNO 5的全部，MMU 11到RNO 14的远端区域(14q21-q22)，MMU 7和MMU 19都到RNO 1，从1q21到41(MMU 7)和1q42到端粒(MMU 19)，以及MMU X到RNO X的全部。根据观察到的跨物种杂交模式，结合已知的小鼠或大鼠基因定位数据，预测了几个新的小鼠和大鼠基因图谱分配。
Mouse and rat genome studies are vital to the use of rodents as models of biology and human genetic disease. In this study, comparative cytogenetic maps of individual homologous mouse (Mus musculus) and rat (Rattus norvegicus) chromosomal regions are presented as defined by cross-species fluorescencein situhybridization. Such “Zoo-FISH” methods permit direct visual observation of the location of DNA segments from one species on mitotic chromosomes of evolutionarily diverged species. Mouse whole chromosome paint (WCP) probes generated from microdissection and degenerate oliogonucleotide primed (DOP) PCR were hybridized on slides containing a mixture of both mouse (the reference species) and rat (the diverged/comparative species) metaphase chromosomes. Using six different mouse WCPs, eight regions on seven rat chromosomes were shown to be evolutionarily conserved between these species. The specific chromosomal sites of homology delineated in this study between mouse (MMU) and rat (RNO) genomes include the following: MMU 1 to RNO 9q21–q36 and to RNO 13 from bands q11 to the telomere, MMU 4 to all of RNO 5, MMU 11 to all of RNO 10 and the distal region of RNO 14 (14q21–q22), MMU 7 and MMU 19 both to RNO 1, from bands 1q21 to 41 (MMU 7) and 1q42 to the telomere (MMU 19), and MMU X to all of RNO X. Additionally, several new mouse and rat map assignments have been predicted based on the observed cross-species hybridization patterns in conjunction with known mapping data for mouse or rat genes.