The effect of selenium and vitamin E on the lymphocytes and immunoglobulin-containing plasma cells in the lymphoid organ and mucosa-associated lymphatic tissues of broiler chickens
The effect of selenium and vitamin E on the lymphocytes and immunoglobulin-containing plasma cells in the lymphoid organ and mucosa-associated lymphatic tissues of broiler chickens
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DOI：
10.1111/j.1439-0264.2007.00799.x
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发表时间：
2008-02-01
影响因子：
0.9
通讯作者：
Kon, Y.
中科院分区：
文献类型：
--
作者：
Khan, M. Z. I.;Akter, S. H.;Kon, Y.
本研究旨在了解硒和维生素E对Kasilla肉鸡不同出生阶段淋巴器官和回肠(黏膜相关淋巴组织的代表器官)中淋巴细胞和含Ig浆细胞频率的影响。用常规苏木精-伊红(H和E)染色方法研究淋巴细胞变化的组织学，用间接免疫过氧化物酶染色方法研究含Ig的浆细胞在对照肉鸡淋巴组织和回肠内的分布和动态变化，以及在日粮中添加不同浓度的硒和维生素E的肉鸡。在组织学上，0.1 mg和0.5 mg补硒组肉鸡胸腺小叶、法氏囊髓质、脾肿块、盲肠扁桃体淋巴小结和髂骨绒毛中淋巴细胞数量较对照组减少。本研究发现，在添加150 mg和300 mg维生素F的鸡中，这些细胞的数量增加。在脾组织中，含免疫球蛋白G和免疫球蛋白M的浆细胞多于含免疫球蛋白A的浆细胞。在盲肠扁桃体和回肠中，含IgA的浆细胞多于含Ig G和Ig M的浆细胞。补硒0.1 mg和0.5 mg组免疫阳性细胞减少，添加150 mg和300 mg维生素E组免疫阳性细胞频率增加。在脾、盲肠扁桃体和回肠中，含IgM的浆细胞的频率在补硒后减少更多，并通过补充维生素E恢复其种群。
The present research has been designed to understand the effect of selenium and vitamin E on the lymphocyte and changes in the frequency of Ig-containing plasma cells in the lymphatic organ and ileum (representative organ for mucosa-associated lymphatic tissues) of different postnatal stages of Kasilla broiler chickens. A routine haematoxylin and eosin (H and E) stain were used to study the histology of the lymphocytic changes, and indirect immunoperoxidase staining method was performed for the study of the distributional and dynamical changes of the Ig-containing plasma cells within the lymphatic tissues and in the ileum of control broilers and in the broilers supplemented with different concentration of selenium and vitamin E in the diet. Histologically, the population of lymphocytes decreased in the lobules of the thymus, medulla of bursal follicles, splenic masses, lymphatic nodules of the cecal tonsil, and villi of the ilium in 0.1 mg and 0.5 mg selenium supplemented broilers in comparison with the control. The population of these cells was found to increase in 150 mg and 300 mg vitamin F supplemented chickens in the present study. In the spleen IgG- and the IgM-containing plasma cells were more than IgA-containing plasma cells. In contrast, in the cecal tonsil and ileum IgA-containing plasma cells were more than IgG- and IgM-containing plasma cells. The frequency of these immunopositive cells were decreased in 0.1 mg and 0.5 mg selenium supplementated chickens, and increased their frequency in the chickens supplemented with 150 mg and 300 mg vitamin E. In the spleen the frequency of IgM-containing plasma cells and both in the cecal tonsil and ileum, the IgG-containing plasma cells were more decreased by selenium supplementation which restored in their population by vitamin E supplementation.