Cell type-specific negative regulatory element in the control region of the rat alpha-fetoprotein gene.
Cell type-specific negative regulatory element in the control region of the rat alpha-fetoprotein gene.
复制标题
大鼠甲胎蛋白基因控制区的细胞类型特异性负调控元件。
DOI：
10.1073/pnas.83.20.7653
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Rothman-Denes,LB
中科院分区：
文献类型：
--
作者：
Muglia,L;Rothman-Denes,LB
白蛋白和甲胎蛋白是进化上相关的基因，在发育过程中表现出差异和复杂的调控模式。我们研究了大鼠甲胎蛋白基因转录起始位点侧翼序列在肝细胞和非肝细胞系瞬时转染试验中的作用。嵌合侧翼区和缺失分析已经确定了以下三个功能不同的区域：细胞类型特异性增强子，包括3个位于-7个双酶对和-4个双酶对之间的双酶;细胞类型特异性启动子，在没有增强子的情况下无活性，并且在转录起始位点上游包含至多180个碱基对;和位于启动子和增强子之间的550个碱基对区域（在~ 3.5个碱基处），以细胞类型特异性方式下调转录起始，并且也能够抑制异源启动子。这些研究结果的影响方面的复杂模式的AFP监管进行了讨论。
Albumin and alpha-fetoprotein are evolutionarily related genes that show differential and complex patterns of regulation during development. We investigated the role of the sequences flanking the transcription initiation site of the rat alpha-fetoprotein gene in transient transfection assays of hepatic and nonhepatic cell lines. Chimeric flanking regions and deletion analysis have defined the following three functionally different regions: a cell type-specific enhancer(s), encompassing 3 kilobases, located between -7 kilobase pairs and -4 kilobase pairs; a cell type-specific promoter, inactive in the absence of an enhancer and comprising at most the 180 base pairs upstream from the site of transcription initiation; and a 550-base-pair region, located between the promoter and the enhancer (at -3.5 kilobases), down-regulates transcription initiation in a cell type-specific manner and is also capable of repressing heterologous promoters. The implications of these findings with respect to the complex pattern of AFP regulation are discussed.