Bacillus cereus electron transport and proton motive force during aerotaxis.
Bacillus cereus electron transport and proton motive force during aerotaxis.
复制标题
蜡状芽孢杆菌趋航过程中的电子传递和质子动力。
DOI：
10.1128/jb.159.3.820-824.1984
复制
发表时间：
1984
影响因子：
3.2
通讯作者：
Taylor,BL
中科院分区：
文献类型：
--
作者：
Laszlo,DJ;Niwano,M;Goral,WW;Taylor,BL
蜡样芽孢杆菌 M63（一种运动菌株）的趋气性（向氧迁移）被氰化钾和 2-庚基-4-羟基喹啉 N-氧化物抑制，表明需要电子传递系统的末端氧化酶（细胞色素 aa3）和细胞色素 b 片段。产生半最大气动反应 (K0.5) 的氧气浓度为 0.31 µM，与呼吸的 Km (0.80 µM) 相似。当厌氧细胞通气时，质子动力从-135增加到-177 mV，并且提出趋气信号是由于呼吸增加而导致的质子动力的增加。本研究最初使用的蜡状芽孢杆菌 T 菌株是不动的，生长为长链细胞，并且缺乏自溶酶。 B. cereus M63 是具有正常运动性的 B. cereus T 的自发衍生物。
Aerotaxis (migration towards oxygen) of Bacillus cereus M63, a motile strain, was inhibited by potassium cyanide and 2-heptyl-4-hydroxyquinoline N-oxide, indicating a requirement for both the terminal oxidase (cytochrome aa3) and the cytochrome b segment of the electron transport system. The concentration of oxygen that gave a half-maximal aerotactic response (K0.5) was 0.31 microM, which was similar to the Km for respiration (0.80 microM). The proton motive force increased from -135 to -177 mV when anaerobic cells were aerated, and it is proposed that the signal for aerotaxis is the increase in proton motive force that results from increased respiration. A strain of B. cereus T initially used in this study was immotile, grew as long chains of cells, and was deficient in autolytic enzyme. B. cereus M63 is a spontaneous derivative of B. cereus T that has normal motility.