Synthesis of Rauscher murine leukemia virus-specific polypeptides in vitro.
Synthesis of Rauscher murine leukemia virus-specific polypeptides in vitro.
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Rauscher鼠白血病病毒特异性多肽的体外合成。
DOI：
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发表时间：
1976
影响因子：
11.1
通讯作者：
H. Bloemendal
中科院分区：
文献类型：
--
作者：
A. Gielkens;D. V. Zaane;H. P. Bloemers;H. Bloemendal
在兔网织红细胞裂解物中研究了由来自感染Rauscher白血病病毒的JLS-V9细胞的纯化病毒信使RNA指导的特异性多肽的生物合成。35 S病毒mRNA产生两种分子量为65，000和72，000的主要产物。还在来源于感染细胞的裂解物中研究了特异性多肽的合成。主要产物是分子量为65，000、76，000和82，000的多肽，并且优先与膜结合制备。在补充有纯化的多聚核糖体的无细胞系统中合成的65，000分子量的病毒特异性多肽的相对含量对于膜结合的多聚核糖体来说相当高。
The biosynthesis of specific polypeptides directed by purified viral messenger RNA from JLS-V9 cells infected with Rauscher leukemia virus has been studied in a rabbit reticulocyte lysate. The 35S viral mRNA gives rise to two major products of 65,000 and 72,000 molecular weight. The synthesis of specific polypeptides was also investigated in lysates derived from infected cells. The main products were polypeptides with molecular weights of 65,000, 76,000, and 82,000, and were preferentially made in association with membranes. The relative content of the virus-specific polypeptide of 65,000 molecular weight, synthesized in a cell-free system supplemented with purified polyribosomes, is considerably higher for membrane-bound polyribosomes.