Increased Plasma Levels of Select Deoxy-ceramide and Ceramide Species are Associated with Increased Odds of Diabetic Neuropathy in Type 1 Diabetes: A Pilot Study.
Increased Plasma Levels of Select Deoxy-ceramide and Ceramide Species are Associated with Increased Odds of Diabetic Neuropathy in Type 1 Diabetes: A Pilot Study.
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DOI：
10.1007/s12017-016-8423-9
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发表时间：
2017-03
影响因子：
3.5
通讯作者：
DCCT/EDIC Group of Investigators
中科院分区：
文献类型：
--
作者：
Hammad SM;Baker NL;El Abiad JM;Spassieva SD;Pierce JS;Rembiesa B;Bielawski J;Lopes-Virella MF;Klein RL;DCCT/EDIC Group of Investigators
2型糖尿病患者血浆脱氧鞘氨醇碱升高，与糖尿病远端感觉运动性多发性神经病的分期相关;然而，脱氧鞘脂（DSL）与1型糖尿病神经病变之间的相关性尚未研究。这项探索性初步研究的主要目的是评估DCCT/EDIC 1型糖尿病子队列中多种鞘脂物质（包括DSL和游离氨基酸）与症状性神经病变之间的相关性。使用质谱，血浆DSL和游离氨基酸的DCCT/EDIC 1型糖尿病参与者（n = 80），有和没有神经病变的症状，进行了调查。患者确定的神经病变是基于15项自我管理的问卷（密歇根神经病变筛查仪），开发用于评估糖尿病患者的远端对称性周围神经病变。评分≥4分或报告在行走时无法感觉到自己的脚或在洗澡时无法区分热水和冷水的患者被认为是神经病性的。测定血浆神经酰胺、鞘磷脂、己糖基和乳糖基神经酰胺以及氨基酸水平，并分析其与患者神经病变状态的相关性。有神经病变的患者脱氧-C24-神经酰胺、C24-和C26-神经酰胺高于无神经病变的患者。半胱氨酸在神经病变患者中较高。未检测到其他鞘脂或氨基酸的差异。患者报告的阳性神经病变的协变量校正比值比与脱氧-C24-和脱氧-C24：1-神经酰胺; C22-、C24-和C26-神经酰胺;以及半胱氨酸水平升高相关。血浆脱氧神经酰胺和神经酰胺种类可能对糖尿病神经病变具有潜在的诊断和预后意义。
Plasma deoxy-sphingoid bases are elevated in type 2 diabetes patients and correlate with the stage of diabetic distal sensorimotor polyneuropathy; however, associations between deoxy-sphingolipids (DSL) and neuropathy in type 1 diabetes have not been examined. The primary aim of this exploratory pilot study was to assess the associations between multiple sphingolipid species including DSL and free amino acids and the presence of symptomatic neuropathy in a DCCT/EDIC type 1 diabetes subcohort. Using mass spectroscopy, plasma levels of DSL and free amino acids in DCCT/EDIC type 1 diabetes participants (n = 80), with and without symptoms of neuropathy, were investigated. Patient-determined neuropathy was based on 15-item self-administered questionnaire (Michigan Neuropathy Screening Instrument) developed to assess distal symmetrical peripheral neuropathy in diabetes. Patients who scored ≥4, or reported inability to sense their feet during walking or to distinguish hot from cold water while bathing were considered neuropathic. Plasma levels of ceramide, sphingomyelin, hexosyl- and lactosylceramide species, and amino acids were measured and analyzed relative to neuropathy status in the patient. Deoxy-C24-ceramide, C24- and C26-ceramide were higher in patients with neuropathy than those without neuropathy. Cysteine was higher in patients with neuropathy. No differences in other sphingolipids or amino acids were detected. The covariate-adjusted Odds Ratios of positive patient-reported neuropathy was associated with increased levels of deoxy-C24-, and deoxy-C24:1-ceramide; C22-, C24-, and C26-ceramide; and cysteine. Plasma deoxy-ceramide and ceramide species may have potential diagnostic and prognostic significance in diabetic neuropathy.
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DOI：
10.1074/jbc.r115.658823
发表时间：
2015-06-19
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Duan J;Merrill AH Jr
通讯作者：
Merrill AH Jr
影响因子：
3
作者：
Schoffski, P.;Dumez, H.;Giaccone, G.
通讯作者：
Giaccone, G.
影响因子：
6.5
作者：
Silva, Liana C.;Ben David, Oshrit;Futerman, Anthony H.
通讯作者：
Futerman, Anthony H.
影响因子：
9.8
作者：
Rotthier, Annelies;Auer-Grumbach, Michaela;Timmerman, Vincent
通讯作者：
Timmerman, Vincent
影响因子：
3.4
作者：
Massard, C.;Salazar, R.;Soria, J. C.
通讯作者：
Soria, J. C.