Bcl6aa and bcl6ab are ubiquitously expressed and are inducible by lipopolysaccharide and polyI:C in adult tissues of medaka Oryzias latipes
Bcl6aa and bcl6ab are ubiquitously expressed and are inducible by lipopolysaccharide and polyI:C in adult tissues of medaka Oryzias latipes
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Bcl6aa 和 bcl6ab 在青鳉成体组织中普遍表达，并可被脂多糖和 PolyI:C 诱导
DOI：
10.1002/jez.b.22843
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Zhao Haobin
中科院分区：
文献类型：
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作者：
Zhang Runshuai;Wu Kongyue;Ke Xiaomei;Zhang Xueyan;Xu Gongyu;Shen Hao;Nibona Emile;Al Hafiz Abdullah;Liang Xiaoting;Wang Zequn;Qi Chao;Zhou Qingchun;Zhong Xueping;Zhao Haobin
B细胞淋巴瘤6 （Bcl6）是一种转录抑制因子，在哺乳动物的先天性和适应性免疫反应、淋巴细胞分化和细胞周期调节等多种生理活动中发挥重要作用。Bcl6a的两个同源物，即Bcl6aa和Bcl6ab，已在包括medakoryzias latipes在内的硬骨鱼中被鉴定出来。利用逆转录聚合酶链反应和原位杂交技术研究了bcl6aa和bcl6abin medaka的表达谱。bcl6aab和bcl6abb的转录本从非常早期的胚胎（如四细胞阶段）到孵化都被检测到。通过原位杂交，从受精后5天开始，bcl6aab和bcl6abb在胚胎体中被清晰地检测到。bcl6aaa在视网膜中特异性表达，而bcl6aba在整个胚体中表达。结果表明，bcl6a和bcl6均来自母系受精卵，并可能在medaka的胚胎发生中起主要作用。在所有被检查的成人组织中，包括免疫器官，如鳃、脾、肾、肝和肠，都检测到bcl6aab和bcl6abb的转录本。脂多糖和多核糖苷-多核糖苷可上调或下调bcl6aaa和bcl6abin在肝脏、脾脏、头肾和肠道中的表达。这些结果表明bcl6aab和bcl6abb都可能参与medaka的免疫应答。
B‐cell lymphoma‐6 (Bcl6) is a transcriptional repressor that plays important roles in various physiological activities such as innate and adaptive immune response, lymphocyte differentiation, and cell cycle regulation in mammals. Two homologs of Bcl6a, namely Bcl6aa and Bcl6ab, are identified in teleost fish including medakaOryzias latipes. The expression profiles ofbcl6aaandbcl6abin medaka were studied using reverse‐transcription polymerase chain reaction and in situ hybridization. The transcripts ofbcl6aaandbcl6abwere detected from very early embryos such as the four‐cell stage until hatching.Bcl6aaandbcl6abwere clearly detected in the embryonic body from 5 days postfertilization onward by in situ hybridization.Bcl6aawas specifically expressed in the retina, whereasbcl6abwas expressed in entire embryonic body. The results referred to that bothbcl6aaandbcl6aboriginate maternally in the zygotes and may play major roles in embryogenesis of medaka. The transcripts ofbcl6aaandbcl6abwere detected in all examined adult tissues, including immune organs such as the gill, spleen, kidney, liver, and intestine. The expression ofbcl6aaandbcl6abin the liver, spleen, head–kidney, and intestine could be upregulated or downregulated by lipopolysaccharide and polyriboinosinic–polyribocytidylic acid. These results indicate that bothbcl6aaandbcl6abmay be involved in immune response in medaka.