Expression of mouse telomerase catalytic subunit in embryos and adult tissues
Expression of mouse telomerase catalytic subunit in embryos and adult tissues
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DOI：
10.1073/pnas.95.18.10471
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发表时间：
1998-09-01
影响因子：
11.1
通讯作者：
Blasco, MA
中科院分区：
文献类型：
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作者：
Martin-Rivera, L;Herrera, E;Blasco, MA
端粒酶是延长端粒的核糖核蛋白复合物，允许在多次细胞分裂期间稳定维持染色体。在这里，我们描述了小鼠端粒酶催化亚基mTERT的分离和表征（小鼠端粒酶逆转录酶），端粒酶复合体的一种必需蛋白组分在胚胎发育过程中，mTERTmRNA在整个胚胎中大量表达，特别是在强烈增殖的区域，我们发现，胚胎和成人组织中的mTERTmRNA表达与端粒酶的基本RNA组分mTR无关，因此，在活性端粒酶复合物的形成中，mTERT蛋白仅存在于具有端粒酶活性的组织中，如睾丸、脾和胸腺。在mTR（-/-）小鼠的胸腺中几乎检测不到mTERT蛋白，这表明该组织中mTERT蛋白的稳定性可能取决于活性端粒酶复合物的实际组装。最后，我们发现小鼠和人的端粒酶催化亚基定位于细胞核，并且其定位在细胞周期进程中不受调节。
Telomerase is a ribonucleoprotein complex that elongates telomeres, allowing the stable maintenance of chromosomes during multiple cell divisions. Here, we describe the isolation and characterization of the catalytic subunit of mouse telomerase, mTERT (mouse telomerase reverse transcriptase), an essential protein component of the telomerase complex During embryonic development, mTERT mRNA is abundantly expressed in the whole embryo, especially in regions of intense proliferation, We found that the mTERT mRNA expression in both embryonic and adult tissues is independent of the essential RNA component of telomerase, mTR, and therefore, of the formation of active telomerase complexes, mTERT protein is present exclusively in tissues with telomerase activity, such as testis, spleen, and thymus. mTERT protein is barely detectable in the thymus of mTR(-/-) mice, suggesting that mTERT protein stability in this tissue may depend on the actual assembly of active telomerase complexes. Finally, we found that mouse and human telomerase catalytic subunit is located in the cell nucleus, and its localization is not regulated during cell cycle progression.