Effect of perfluorodecalin as an oxygen carrier on actinorhodin production by Streptomyces coelicolor A3(2)
Effect of perfluorodecalin as an oxygen carrier on actinorhodin production by Streptomyces coelicolor A3(2)
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DOI：
10.1007/bf00172813
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发表时间：
1995-05
影响因子：
5
通讯作者：
M. Elibol;F. Mavituna
中科院分区：
文献类型：
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作者：
M. Elibol;F. Mavituna
全氟萘烷是一种全氟化碳（PFC），在本研究中被用作天蓝色链霉菌培养基中的溶解氧载体。在摇瓶培养中，使用确定的和复合的培养基，研究了不同浓度的PFC，PFC与普朗尼克F-68乳化和普朗尼克单独的影响。在限定培养基中，与PFC单独，最大生物量和放线菌紫素浓度和体积底物消耗率增加与PFC浓度的增加。然而，当PFC浓度超过50%（v/v）时，它们急剧下降。用普朗尼克F-68乳化PFC导致抗生素浓度显著增加，而生长不受影响。在发酵培养基中单独包含超过4g/l的普朗尼克抑制生长。在含有40%（v/v）PFC的复合培养基中，虽然最终抗生素浓度不受影响，但放线菌紫素积累的开始时间比对照提前2天。结果表明，PFC和乳化PFC对S没有任何有害影响。腔肠色彩文化
Perfluorodecalin, a perfluorocarbon (PFC), was used in this investigation as a dissolved oxygen carrier in the media ofStreptomyces coelicolorcultures. The effects of different concentrations of PFC, PFC emulsified with pluronic F-68 and pluronic alone were investigated in the shake-flask cultures using both defined and complex media. In the defined medium with PFC alone, the maximum biomass and actinorhodin concentrations and the volumetric substrate consumption rates increased with increasing PFC concentration. They decreased dramatically, however, when the PFC concentration exceeded 50% (v/v). Emulsifying the PFC with pluronic F-68 resulted in a significant increase in antibiotic concentration while growth was unaffected. The inclusion of more than 4 g/l pluronic alone in the fermentation medium inhibited the growth. In the complex medium with 40% (v/v) PFC, although the final antibiotic concentration was unaffected, the onset of actinorhodin accumulation was 2 days earlier than that in the control. It was demonstrated that PFC and emulsified PFC did not have any deleterious effects onS. coelicolorcultures.