Identification of tumor microenvironment-related prognostic genes in colorectal cancer based on bioinformatic methods.
Identification of tumor microenvironment-related prognostic genes in colorectal cancer based on bioinformatic methods.
复制标题
DOI：
10.1038/s41598-021-94541-6
复制
发表时间：
2021-07-22
影响因子：
4.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Liu Y;Cheng L;Li C;Zhang C;Wang L;Zhang J
结直肠癌（CRC）在全球最致命的癌症中排名第四，其进展受到肿瘤微环境（TME）的高度影响。本研究探讨了TME和结直肠癌预后之间的关系，并确定了与CRC微环境相关的预后基因。我们收集了癌症基因组图谱（TCGA）中的基因表达数据，并通过ESTIMATE算法计算了结直肠癌中基质/免疫细胞的评分及其与临床结局的关系。较低的免疫评分与CRC的恶性进展显著相关（转移，p = 0.001）。我们通过将CRC病例分为高和低基质/免疫评分组筛选了292个差异表达基因（DEG）。功能富集分析和蛋白质-蛋白质相互作用（PPI）网络表明，这些DEG与免疫应答、细胞因子受体相互作用和趋化因子信号通路密切相关。通过生存分析确定了六种具有预后价值的DEG（FABP 4、MEOX 2、MMP 12、ERMN、TNFAIP 6和CHST 11），并在两个独立队列（GSE 17538和GSE 161158）中进行了验证。基于肿瘤免疫评估资源（TIMER），6个DEG与免疫细胞浸润水平显著相关。这些结果可能有助于发现新的诊断和预后生物标志物和新的治疗靶点。
Colorectal cancer (CRC) ranks fourth among the deadliest cancers globally, and the progression is highly affected by the tumor microenvironment (TME). This study explores the relationship between TME and colorectal cancer prognosis and identifies prognostic genes related to the CRC microenvironment. We collected the gene expression data from The Cancer Genome Atlas (TCGA) and calculated the scores of stromal/immune cells and their relations to clinical outcomes in colorectal cancer by the ESTIMATE algorithm. Lower immune scores were significantly related to the malignant progression of CRC (metastasis, p = 0.001). We screened 292 differentially expressed genes (DEGs) by dividing CRC cases into high and low stromal/immune score groups. Functional enrichment analyses and protein–protein interaction (PPI) networks illustrated that these DEGs were closely involved in immune response, cytokine–cytokine receptor interaction, and chemokine signaling pathway. Six DEGs (FABP4, MEOX2, MMP12, ERMN, TNFAIP6, and CHST11) with prognostic value were identified by survival analysis and validated in two independent cohorts (GSE17538 and GSE161158). The six DEGs were significantly related to immune cell infiltration levels based on the Tumor Immune Estimation Resource (TIMER). The results might contribute to discovering new diagnostic and prognostic biomarkers and new treatment targets for colorectal cancer.
登录
查看更多内容
影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
DOI：
10.1007/978-3-030-36214-0_1
发表时间：
2020-01-01
期刊：
TUMOR MICROENVIRONMENTS IN ORGANS: FROM THE BRAIN TO THE SKIN - PT A
影响因子：
--
作者：
Leman, J. K. H.;Munoz-Erazo, L.;Kemp, R. A.
通讯作者：
Kemp, R. A.
DOI：
10.1016/j.urolonc.2018.12.009
发表时间：
2019-04-01
影响因子：
2.7
作者：
Chan, Ti-Chun;Li, Chien-Feng;Li, Wei-Ming
通讯作者：
Li, Wei-Ming
影响因子：
7.5
作者：
Ge, Penglei;Wang, Weiwei;Wu, Yang
通讯作者：
Wu, Yang
与肿瘤微环境相关的九个基因特征可预测卵巢癌的总体生存率
影响因子：
5.2
作者：
Ding, Qi;Dong, Shanshan;Zeng, Yong
通讯作者：
Zeng, Yong