RHODOPSIN CONTENT IN OUTER SEGMENT MEMBRANES OF BOVINE AND FROG RETINAL RODS
RHODOPSIN CONTENT IN OUTER SEGMENT MEMBRANES OF BOVINE AND FROG RETINAL RODS
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DOI：
10.1021/bi00708a031
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
DREYER, WJ
中科院分区：
文献类型：
--
作者：
PAPERMASTER, DS;DREYER, WJ
材料和方法新鲜牛眼来自当地一家屠宰场(优质肉类包装，加利福尼亚州弗农)。并在斩首后的0.5小时内保存在黑暗的冰面上。偶尔会获得400只眼睛，在这种条件下，一些眼睛已经保存了长达6小时。冰冻视网膜(明尼苏达州奥斯汀霍梅尔)体重50-150克(康涅狄格山谷公司)的北美罐头林蛙通过食道插管用四环素治疗5天，以防止败血症(Gibbs等人，1966)。在使用之前，青蛙需要进行一夜的黑暗适应。均来自康涅狄格州纽黑文耶鲁医学院病理学系06510(D.S.P.)和加利福尼亚州帕萨迪纳市加州理工学院生物学部91109(W.J.D.)。1973年9月14日收到。美国癌症协会和美国公共卫生服务特别奖学金CA 50645的PF527博士后奖学金获得者。这项工作得到了美国公共卫生服务GRAM GM 06965和RR 05358以及安娜·富勒基金拨款382-1的支持。使用的缩写是：ROS，杆件外节。
Materials and MethodsFresh cattle eyes were obtained from a local slaughter-house (Quality Meat Packing, Vernon, Calif.) and stored in darkness on ice within 0.5 hr of decapitation. Occasionally, 400 eyes were obtained and under these conditions some of the eyes had been stored for as long as 6 hr. Frozen retinas (Hormel, Austin, Minn.) were stored at—20. North Ameri-can Rana pipiens, weighing 50-150 g (Connecticut Valley Corp.), were treated with tetracycline for 5 days by esophageal intubation in order to prevent sepsis (Gibbs et al., 1966). Frogs were dark adapted overnight prior to use. All sub-t From the Department of Pathology, Yale Medical School, New Haven, Connecticut 06510 (D. S. P.), and the Division of Biology, California Institute of Technology, Pasadena, California 91109 (W. J. D.). Received September 14, 1973. t Recipient of Postoctoral Fellowship PF 527 from the American Cancer Society and U. S. Public Health Service Special Fellowship CA 50645. This work was supported by the U. S. Public Health Service Grams GM 06965 and RR 05358 and Anna Fuller Fund Grant 382-1.! Abbreviation used is: ROS, rod outer segments.