Biochemical and Biophysical Comparison of Native and Chemically Synthesized Phospholamban and a Monomeric Phospholamban Analog (*)
Biochemical and Biophysical Comparison of Native and Chemically Synthesized Phospholamban and a Monomeric Phospholamban Analog (*)
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天然和化学合成的受磷班和单体受磷班类似物的生化和生物物理比较 (*)
DOI：
--
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发表时间：
1996
影响因子：
4.8
通讯作者：
Robert G. Johnson
中科院分区：
文献类型：
--
作者：
Ernest J. Mayer;E. McKenna;V M Garsky;Carl J. Burke;H. Mach;C. Middaugh;M. Sardana;Jeffrey S. Smith;Robert G. Johnson
Phospholamban (PLB) was rapidly isolated from canine cardiac sarcoplasmic reticulum using immunoaffinity chromatography and prepared by solid phase peptide synthesis. The two proteins are indistinguishable when analyzed by SDS-polyacrylamide gel electrophoresis and exhibit pentameric oligomeric states. They are similarly detected on Western blots, are phosphorylation substrates, have identical amino acid compositions that directly reflect their predicted values, yield the same internal amino acid sequences upon CNBr digestion, and have molecular mass values agreeing with the expected value (6123 Da). Native and synthetic PLB reduced the calcium sensitivity of CaATPase, which is reversed by anti-PLB antibody. A Cys-to-Ser PLB analog, where the cysteines (36, 41, and 46) were substituted by serines, is monomeric on SDS-polyacrylamide gel electrophoresis, can be phosphorylated, and is recognized by polyclonal antisera. PLB migrates with a sedimentation coefficient of 4.8 S in sedimentation velocity ultracentrifugation experiments, whereas Cys-to-Ser PLB does not sediment, consistent with a monomeric state. Circular dichroism spectral analysis of PLB indicates about 70% α-helical structure, whereas Cys-to-Ser PLB manifests only about 30%. Because the physiochemical properties of native and synthetic PLB appear identical, the more readily available synthetic protein should be suitable for more extensive structural studies.
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影响因子：
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作者：
Hymel,L;Fleischer,S
通讯作者：
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DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Toyofuku,T;Kurzydlowski,K;Lytton,J;MacLennan,DH
通讯作者：
MacLennan,DH
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DOI：
10.1016/0167-4838(89)90203-3
发表时间：
1989
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
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通讯作者：
Jones,LR
影响因子：
2.9
作者：
Kirchberger,MA;Borchman,D;Kasinathan,C
通讯作者：
Kasinathan,C
影响因子：
2.9
作者：
SREERAMA, N;WOODY, RW
通讯作者：
WOODY, RW