Hepatic hypoperfusion after intestinal reperfusion.
Hepatic hypoperfusion after intestinal reperfusion.
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DOI：
10.1016/s0039-6060(96)80163-2
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发表时间：
1996-02
期刊：
影响因子：
3.8
通讯作者：
R. Turnage;R. Turnage;R. Turnage;K. Kadesky;K. Kadesky;K. Kadesky;Stuart I. Myers;Stuart I. Myers;Stuart I. Myers;K. Guice;K. Guice;K. Guice;Keith T. Oldham;Keith T. Oldham;Keith T. Oldham
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文献类型：
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作者：
R. Turnage;R. Turnage;R. Turnage;K. Kadesky;K. Kadesky;K. Kadesky;Stuart I. Myers;Stuart I. Myers;Stuart I. Myers;K. Guice;K. Guice;K. Guice;Keith T. Oldham;Keith T. Oldham;Keith T. Oldham
背景肠缺血再灌注损伤（intestinal ischemia-reperfusion injury，IIR）可导致肝、肺功能障碍，因此常被用作多器官功能衰竭综合征的模型。本研究探讨的假设，肝血流量显着减少，在此injury model.MethodsSprague-Dawley大鼠进行了120分钟的肠缺血和60分钟的再灌注（IIR）。用放射性标记微球和多普勒血流探针测量肝血流量。通过测定胆汁流量、血清丙氨酸氨基转移酶和肝组织三磷酸腺苷水平来定量肝功能障碍。假手术动物作为controls.ResultsIntestinal缺血减少门静脉流量66%，与假手术动物相比（p=0.0001），但对肝动脉流量没有影响。相比之下，与对照组相比，再灌注使肝动脉血流量减少了80%（p=0.002），其中大部分变化发生在再灌注后5分钟内。IIR引起胆汁流量减少63%（p<0.05），血清丙氨酸氨基转移酶水平升高5倍（p<0.0002），肝三磷酸腺苷水平降低33%（p<0.05）。这一观察结果表明，肝缺血可能有助于IIR诱导的肝损伤。
BackgroundIntestinal ischemia-reperfusion injury (IIR) induces hepatic and pulmonary dysfunction and thus has been used as a model of multiple organ failure syndrome. This study examines the hypothesis that hepatic blood flow is markedly reduced in this injury model.MethodsSprague-Dawley rats underwent 120 minutes of intestinal ischemia and 60 minutes of reperfusion (IIR). Hepatic blood flow was measured with radiolabeled microspheres and Doppler flow probes. Hepatic dysfunction was quantitated by measuring bile flow and serum alanine aminotransferase and hepatic tissue adenosine triphosphate levels. Sham-operated animals served as controls.ResultsIntestinal ischemia reduced portal flow by 66% when compared with sham-operated animals (p=0.0001) but had no effect on hepatic arterial flow. In contrast, reperfusion reduced hepatic artery flow by 80% when compared with controls (p=0.002) with most of this change occurring within 5 minutes of reperfusion. IIR induced a 63% reduction in bile flow (p<0.05), a fivefold rise in serum alanine aminotransferase level (p<0.0002), and a 33% reduction in hepatic adenosine triphosphate level (p<0.05).ConclusionsThese data suggest that IIR induces profound hepatic hypoperfusion, which is temporally related to acute hepatic dysfunction. This observation suggests that hepatic ischemia may contribute to IIR-induced liver injury.