Fusarium verticillioides from Sugarcane, Vegetative Compatibillity Groups and Pathogenicity
Fusarium verticillioides from Sugarcane, Vegetative Compatibillity Groups and Pathogenicity
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DOI：
10.17221/49/2011-pps
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发表时间：
2012-06-01
影响因子：
1.3
通讯作者：
Mofrad, Nasrin Noras
中科院分区：
文献类型：
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作者：
Mohammadi, Abbas;Nejad, Reza Farrokhi;Mofrad, Nasrin Noras
从伊朗的主要甘蔗产区收集了受疾病pokkah boeng影响的甘蔗植物样品。从甘蔗上分离到轮枝镰刀菌50个。致病性测定结果表明，所有菌株对甘蔗均具有致病性。从轮枝镰刀菌分离物中产生了485个硝酸盐不利用突变体。用于产生nit突变体的培养基包括马铃薯葡萄糖琼脂、基本培养基和用1.8%、2%、2.5%和3%氯酸钾修正的Czapeck琼脂。Nit突变体分为三个表型（nit1，nit3，和nitM）类的基础上，它们的生长在不同的氮源在培养基中。在测试的分离物中，分别产生280、115和90个nitl、nit3和nitM。Nit突变体被用来迫使异核体的形成，以确定VCG的分布及其与致病性和地理来源的关系。48个F.从甘蔗中分离到轮枝孢属（verticillioides）。46个VCG由单一分离株代表，而其余2个由2个分离株组成。VCG都不常见。
Sugarcane plant samples affected by the disease pokkah boeng were collected from the major sugarcane producing areas in Iran. Fifty isolates of Fusarium verticillioides were recovered from diseased sugarcanes. Results of pathogenicity tests revealed that all isolates were pathogenic to sugarcane. Four hundred and eighty-five nitrate non-utilising mutants were generated from F verticillioides isolates. Media used for nit mutant generation included potato dextrose agar, minimal medium and Czapeck agar amended with 1.8%, 2%, 2.5%, and 3% potassium chlorate. Nit mutants were divided into three phenotypic (nitl, nit3, and nitM) classes based on their growth on different nitrogen sources in the culture media. Among the isolates tested, 280, 115 and 90 nitl, nit3, and nit M were generated, respectively. Nit mutants were used to force the heterokaryon formation to determine the distribution of VCGs and their relation to pathogenicity and geographic origin. Forty-eight VCGs of F. verticillioides were isolated from sugarcane. Forty-six of the VCGs were represented by a single isolate, whereas the remaining two comprised two isolates. None of the VCGs was common.