The development of neutrophilic polymorphonuclear leukocytes in human bone marrow.
The development of neutrophilic polymorphonuclear leukocytes in human bone marrow.
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人骨髓中嗜中性粒细胞多形核白细胞的发展。
DOI：
10.1084/jem.134.4.907
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发表时间：
1971-10-01
影响因子：
15.3
通讯作者：
Farquhar, M G
中科院分区：
文献类型：
--
作者：
Bainton, D F;Ullyot, J L;Farquhar, M G
用组织化学染色、电子显微镜和细胞化学方法对正常人骨髓和血液中的中性粒细胞及其前体进行了检测，以确定其细胞质颗粒的来源和性质。人中性粒细胞包含两种基本类型的颗粒，即中性粒细胞和特异性粒细胞，它们在形态、含量和起源时间上有所不同。嗜氮细胞很大，可能是球形或椭球体，后者带有结晶包体。它们在第一分泌阶段（早髓细胞）产生，含有过氧化物酶和各种溶酶体酶，因此与修饰的原代溶酶体相对应。特异性较小，可呈球形或细长状，形成于分泌后期（髓细胞）。它们缺乏溶酶体酶，含有碱性磷酸酶和碱性蛋白；它们的内容在很大程度上仍未确定。在成熟的中性粒细胞中，特异性粒细胞的数量超过嗜蓝粒细胞，因为在髓细胞阶段细胞分裂时，每个细胞中嗜蓝粒细胞的数量减少。结果表明，就两种类型（嗜氮性和特异性）颗粒的来源、酶活性和在成熟细胞中的持久性而言，兔的情况与前面描述的基本相同。两种PMN的主要区别在于颗粒的形态（大小、形状和密度），尤其是嗜氮细胞。
Neutrophilic leukocytes (PMN) and their precursors from normal human marrow and blood were examined by histochemical staining and by electron microscopy and cytochemistry in order to determine the origin and nature of their cytoplasmic granules. Human neutrophils contain two basic types of granules, azurophils and specifics, which differ in morphology, contents, and time of origin. Azurophils are large and may be spherical or ellipsoid, the latter with a crystalline inclusion. They are produced in the first secretory stage (promyelocyte), contain peroxidase and various lysosomal enzymes, and thus correspond to modified primary lysosomes. Specifics are smaller, may be spherical or elongated, and are formed during a later secretory stage (myelocyte). They lack lysosomal enzymes and contain alkaline phosphatase and basic protein; their contents remain largely undetermined. Specifics outnumber azurophils in the mature PMN because of reduction in numbers of azurophils per cell by cell division in the myelocyte stage. The findings indicate that the situation is basically the same as described previously in the rabbit, insofar as the origin, enzymic activity, and persistence in the mature cell of the two types (azurophil and specific) of granules are concerned. The main difference between PMN of the two species is in the morphology (size, shape, and density) of the granules, especially the azurophils.