Plane wave analysis of coherent holographic image reconstruction by phase transfer (CHIRPT)
Plane wave analysis of coherent holographic image reconstruction by phase transfer (CHIRPT)
复制标题
DOI：
10.1364/josaa.32.002156
复制
发表时间：
2015-11-01
影响因子：
1.9
通讯作者：
Bartels, Randy A.
中科院分区：
文献类型：
--
作者：
Field, Jeffrey J.;Winters, David G.;Bartels, Randy A.
荧光成像在无数的科学努力中起着关键作用，特别是在生物科学中。荧光强度的三维成像通常需要串行数据采集，也就是说，体素的体素收集整个标本发出的荧光与非成像单元素检测器。虽然非成像荧光检测提供了一些散射鲁棒性的措施，在动态标本可以成像的速度是严重限制。其他荧光成像技术利用成像检测来提高收集率。一个值得注意的例子是光片荧光显微镜，也被称为选择性平面照明显微镜，它照亮了一个大的区域内的标本和收集发射的荧光在一个角度垂直或倾斜的照明光片。不幸的是，所发射的荧光的散射可能导致在高度混浊的生物介质中所收集的图像的模糊。我们最近介绍了一种成像技术，称为相干全息图像重建相转移（CHIRPT），结合了光片状照明与非成像荧光检测。通过将光片照明的速度与非成像检测的散射鲁棒性相结合，CHIRPT有望对生物成像产生巨大影响，特别是对于体内制备。在这里，我们提出了空间相干照明下的CHIRPT成像的数学形式主义，并提出实验数据，验证了理论模型。(C)2015美国光学学会
Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is, voxel-by-voxel collection of fluorescent light emitted throughout the specimen with a nonimaging single-element detector. While nonimaging fluorescence detection offers some measure of scattering robustness, the rate at which dynamic specimens can be imaged is severely limited. Other fluorescent imaging techniques utilize imaging detection to enhance collection rates. A notable example is light-sheet fluorescence microscopy, also known as selective-plane illumination microscopy, which illuminates a large region within the specimen and collects emitted fluorescent light at an angle either perpendicular or oblique to the illumination light sheet. Unfortunately, scattering of the emitted fluorescent light can cause blurring of the collected images in highly turbid biological media. We recently introduced an imaging technique called coherent holographic image reconstruction by phase transfer (CHIRPT) that combines light-sheet-like illumination with nonimaging fluorescent light detection. By combining the speed of light-sheet illumination with the scattering robustness of nonimaging detection, CHIRPT is poised to have a dramatic impact on biological imaging, particularly for in vivo preparations. Here we present the mathematical formalism for CHIRPT imaging under spatially coherent illumination and present experimental data that verifies the theoretical model. (C) 2015 Optical Society of America