Correction of the DNA Sequence of theregB Gene of Rhodobacter capsulatus with Implications for the Membrane Topology of the Sensor Kinase RegB
Correction of the DNA Sequence of theregB Gene of Rhodobacter capsulatus with Implications for the Membrane Topology of the Sensor Kinase RegB
复制标题
荚膜红杆菌 theregB 基因 DNA 序列的校正及其对传感器激酶 RegB 膜拓扑结构的影响
DOI：
10.1128/jb.182.3.818-820.2000
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发表时间：
2000
影响因子：
3.2
通讯作者：
G. Klug
中科院分区：
文献类型：
--
作者：
Weizhen Chen;A. Jäger;G. Klug
摘要 我们更正了先前发表的 regB 基因序列，该基因编码荚膜红细菌中的组氨酸传感器激酶。推导的 RegB 氨基酸序列在 N 末端有一个额外的推定跨膜结构域。 RegB-PhoA 和 RegB-LacZ 融合蛋白的分析支持具有六个跨膜结构域的 RegB 拓扑模型。
ABSTRACT We corrected the previously published sequence for theregB gene, which encodes a histidine sensor kinase inRhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.