Intraspecific divergences of Rhodiola alsia (Crassulaceae) based on plastid DNA and internal transcribed spacer fragments
Intraspecific divergences of Rhodiola alsia (Crassulaceae) based on plastid DNA and internal transcribed spacer fragments
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基于质体 DNA 和内转录间隔片段的红景天（景天科）种内分歧
DOI：
10.1111/j.1095-8339.2011.01193.x
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发表时间：
2012-02-01
影响因子：
2.4
通讯作者：
Chen, Shilong
中科院分区：
文献类型：
--
作者：
Gao, Qingbo;Zhang, Dejun;Chen, Shilong
本研究利用两个母系遗传的质体DNA基因间隔区rp 120-rps 12和trnS-trnG，以及双亲遗传的核核糖体内转录间隔区（ITS），对青藏高原特有植物高山红景天（Rhodiola alsia，QTP）的遗传变异和地理历史进行了研究。通过对18个居群227个个体的全范围取样，共检测到45种质体DNA单倍型和19种ITS序列类型。只有三个质体DNA单倍型是广泛的，大多数单倍型仅限于单一网站或邻近人群。分子方差分析表明，大部分的遗传变异（51.24%）的群体内发现，但群体也不同（FST = 0.48759）。我们发现了三个具有相对较高的质体DNA多样性的区域，这些区域可以进一步被认为是基于ITS序列类型分布的潜在孤立的分歧中心。这些代表了三个潜在的孤立的冰川避难所R。阿尔西亚：其中一个长期以来被认为是青藏高原东南边缘的重要避难所，而其他人则是新的，位于高原台地上的唐古拉山北部和南部。基于ITS的分歧时间估计表明，R。alsia种内分化距离为0.350.87 Mya，表明更新世气候振荡可能是R.阿尔西亚。高山红景天在第四纪冰期可能经历了一个向孤立的冰川避难所撤退的漫长地理历史，导致了不同程度的异地种内分化。(C)2011年，伦敦林奈学会，林奈学会植物学杂志，2012年，168，204215。
In the present study, we used two maternally inherited plastid DNA intergenic spacers, rpl20-rps12 and trnS-trnG, and the biparentally inherited nuclear ribosomal internal transcribed spacer (ITS) region to explore genetic variation and phylogeographical history of Rhodiola alsia, a herb endemic to the Qinghai-Tibetan Plateau (QTP). Based on range-wide sampling (18 populations and 227 individuals), we detected 45 plastid DNA haplotypes and 19 ITS sequence types. Only three plastid DNA haplotypes were widespread; most haplotypes were restricted to single sites or to neighbouring populations. Analysis of molecular variance revealed that most of the genetic variance was found within populations (51.24%) but that populations were also distinct (FST = 0.48759). We found three areas with relatively high plastid DNA diversity and these could further be recognized as potentially isolated divergence centres based on the ITS sequence type distribution. These represent three potentially isolated glacial refugia for R. alsia: one of them has long been recognized as an important refugium on the south-eastern edge of the QTP, whereas the others are new and located in the north and south of the Tanggula Mountains on the plateau platform. Divergence time estimates based on ITS suggest that the main lineages of R. alsia diverged from each other 0.350.87 Mya, indicating that climatic oscillations during the Pleistocene may have been an important driver of intraspecific divergence in R. alsia. Rhodiola alsia probably experienced a phylogeographical history of retreat to isolated glacial refugia during Quaternary glaciations that led to different degrees of allopatric intraspecific divergence. (C) 2011 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 168, 204215.