Microfluorometric analysis of cellular DNA following incorporation of bromodeoxyuridine
Microfluorometric analysis of cellular DNA following incorporation of bromodeoxyuridine
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掺入溴脱氧尿苷后细胞 DNA 的微荧光分析
DOI：
10.1002/jcp.1040900308
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发表时间：
1977
影响因子：
5.6
通讯作者：
D. Swartzendruber
中科院分区：
文献类型：
--
作者：
D. Swartzendruber
溴脱氧尿苷（BrdU）掺入细胞DNA对几种DNA特异性染料的细胞相关荧光具有不同的影响。用BrdU处理细胞后，用流式显微荧光法研究染色细胞荧光的相对增加或减少。将溴脱氧尿苷掺入CHO细胞中可增加光神霉素、橄榄霉素或色霉素染色细胞的荧光，降低碘化丙啶染色细胞的荧光，并且对吖啶黄Feulgen染色细胞的荧光几乎没有影响（如果有）。细胞相关染料的相对荧光的变化是由于与BrdU取代的DNA结合的染料量的变化。DNA含量的比色法和吸光度测量结果表明，BrdU不改变CHO细胞的二倍体DNA含量，然而，BrdU诱导细胞周期的细胞分布的扰动，导致平均DNA含量的增加。
Bromodeoxyuridine (BrdU) incorporation into cellular DNA has a differential effect on the cell‐associated fluorescence of several DNA‐specific dyes. After cells were treated with BrdU, flow microfluorometry was used to study the relative increase or decrease in fluorescence of stained cells. Bromodeoxyuridine incorporation into CHO cells increased the fluorescence of mithramycin‐, olivomycin‐, or chromomycin‐stained cells, decreased that of propidium iodide‐stained cells, and had little, if any, effect on the fluorescence of acriflavine Feulgen‐stained cells. Changes in relative fluorescence of cell‐associated dyes are due to changes in the amounts of dye bound to cells with BrdU‐substituted DNA. Colorimetric and absorbance measurement of DNA content showed that BrdU does not alter the diploid DNA content of CHO cells; however, BrdU induces perturbations in the distribution of cells about the cell cycle which cause an increase in average DNA content.