Complementary Oligonucleotide Conjugated Multicolor Carbon Dots for Intracellular Recognition of Biological Events.
Complementary Oligonucleotide Conjugated Multicolor Carbon Dots for Intracellular Recognition of Biological Events.
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DOI：
10.1021/acsami.0c02463
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发表时间：
2020-04-08
影响因子：
9.5
通讯作者：
Pan D
中科院分区：
文献类型：
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作者：
Srivastava I;Misra SK;Bangru S;Boateng KA;Soares JANT;Schwartz-Duval AS;Kalsotra A;Pan D
通过使用互补的DNA序列作为表面配体，我们选择性地允许两个单独的扩散“双色”碳量子点在原位和体外相互作用。表面丰富的亚硝基-/硝基-官能团的自发纳米级氧化导致两个不同颜色的碳量子点（CD），其通过极性驱动的色谱分离而分离。发射绿色和红色的碳量子点被互补的单链DNA修饰，所述互补的单链DNA产生相应碳量子点的荧光发射的显著增加。相互胶体相互作用是通过连接到相应颗粒的互补DNA碱基对的杂交实现的，导致其光致发光的淬灭。观察到的杂交后淬灭可能是由于双链体DNA形成后CD的聚集和多色CD的紧密接近的组合效应，具有重叠的光谱区域，导致可能作为热量释放的非辐射能量转移过程。这种策略可能有助于合理设计相互作用的碳量子点，以更好地控制所产生的组装结构，用于研究不同的生物现象，包括分子细胞遗传学。一种新合成的CD成功地用于使用CD的荧光强度（FI）的变化事件来成像GAPDH mRNA的细胞内定位。这种选择性通过将含有互补序列的寡核苷酸与GAPDH mRNA缀合来引入。还发现该缀合的碳点的FI，rCD-GAPDH在Ca 2+存在下降低，并且与H+浓度相关地变化，并且可以用作定量细胞内Ca 2+浓度和pH值（H+）的工具，其可以给出关于细胞存活的重要信息。因此，CD-寡核苷酸缀合物可以作为细胞事件和干预的有效探针。
By using complementary DNA sequences as surface ligands, we selectively allow two individual diffusing ‘dual-color’ carbon dots to interact in situ and in vitro. Spontaneous nanoscale oxidation of surface-abundant nitroso-/nitro- functionalities leads to two distinctly colored carbon dots (CD) which are isolated by polarity driven chromatographic separation. Green and red emitting carbon dots were decorated by complementary single stranded DNAs which produce a marked increase in the fluorescence emission of the respective carbon dots. Mutual colloidal interactions are achieved through hybridization of complementary DNA base pairs attached to the respective particles, resulting in quenching of their photoluminescence. The observed post-hybridization quenching is presumably due to a combined effect from an aggregation of CDs post duplex DNA formation and close proximity of multi-colored CDs, having overlapped spectral regions leading to a non-radiative energy transfer process possibly released as heat. This strategy may contribute to the rational design of mutually interacting carbon dots for a better control over the resulting assembly structure for studying different biological phenomenon including molecular cytogenetics. One of the newly synthesized CDs was successfully used to image intracellular location of GAPDH mRNA using event of change in fluorescence intensity (FI) of CDs. This selectivity was introduced by conjugating an oligonucleotide harboring complementary sequence to GAPDH mRNA. FI of this conjugated carbon dot, rCD-GAPDH was also found to decrease in presence of Ca2+ and varied in relation to H+ concentrations and could serve as a tool to quantify the intracellular concentrations of Ca2+ and pH value (H+) which can give important information about cell survival. Therefore, CD-oligonucleotide conjugates could serve as efficient probes for cellular events and interventions.
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影响因子：
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作者：
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影响因子：
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