Rat polyomavirus 2 infection in a colony of X-linked severe combined immunodeficiency rats in Japan.
Rat polyomavirus 2 infection in a colony of X-linked severe combined immunodeficiency rats in Japan.
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DOI：
10.1292/jvms.18-0107
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发表时间：
2018-09-13
期刊：
影响因子：
--
通讯作者：
Kuramoto T
中科院分区：
文献类型：
--
作者：
Tanaka M;Kuramochi M;Nakanishi S;Kuwamura M;Kuramoto T
多瘤病毒(PYV)感染广泛的动物，并引发消耗性疾病，特别是在免疫抑制的宿主中。最近，在美国一群X连锁的严重联合免疫缺陷(X-SCID)大鼠中发现了一种新的褐家鼠多瘤病毒2(Rattus Norveicus PolyomaVirus 2，RatPyV2)。在这里，我们描述了在日本的一个X-SCID大鼠群体中发现RatPyV2感染的第一个报告。受影响的大鼠表现出成年后的消瘦。组织学上，我们在涎腺、哈德氏腺、眶外泪腺以及呼吸和生殖组织的增生或发育不良的上皮细胞内观察到大的嗜碱性核内包涵体。在这些器官中，腮腺、哈德氏腺和眶外泪腺受到的影响最明显。尤其在1月龄时，腮腺的病变最为严重和弥漫，萎缩性病变明显，提示X-SCID大鼠腮腺对RatPyV2高度易感。RatPyV2包涵体也见于附睾尾和输精管。这种生殖损伤在繁育年龄的后期显著发展，因此可能与感染X-SCID大鼠的生殖力下降有关。我们还在Amp-FTA方法的基础上建立了一种简单、快速、非侵入性的诊断方法，使用口腔拭子检测免疫缺陷大鼠的RatPyV2。我们的发现有助于RatPyV2感染的早期发现和诊断。
Polyomaviruses (PyVs) infect a wide range of animals and provoke wasting diseases, particularly in immunosuppressed hosts. Recently, a novel Rattus norvegicus polyomavirus 2 (RatPyV2) has been identified in a colony of X-linked severe combined immunodeficiency (X-SCID) rats in the United States. Here, we describe the first report of the RatPyV2 infection in an X-SCID rat colony in Japan. The affected rats exhibited adult-onset wasting. Histologically, we observed large basophilic intranuclear inclusion bodies within the hyperplastic or dysplastic epithelial cells in the salivary glands, Harderian glands, extraorbital lacrimal glands, and in respiratory and reproductive tissues. Among these organs, the parotid salivary, Harderian, and extraorbital lacrimal glands were most obviously affected. In particular, the parotid salivary glands were the most severely and diffusely affected and atrophic lesions were prominent even at 1 month of age, which suggested that the parotid salivary glands would be highly susceptible to RatPyV2 in X-SCID rats. RatPyV2 inclusion bodies were also detected in the tail of the epididymis and deferent duct. Such reproductive lesions developed significantly in the later stage of breeding age, and therefore may be associated with the reduced fecundity observed in the infected X-SCID rats. We also established a simple, rapid, and non-invasive diagnostic method based on the Amp-FTA method, using buccal swabs for the detection of RatPyV2 in immunodeficient rats. Our findings contribute to the early detection and diagnosis of RatPyV2 infections.
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