Absolute Determination Method Using Chirality for Glufosinate and Bialaphos by γ-Cyclodextrin Modified Capillary Zone Electrophoresis
Absolute Determination Method Using Chirality for Glufosinate and Bialaphos by γ-Cyclodextrin Modified Capillary Zone Electrophoresis
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DOI：
10.2116/analsci.21.31
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发表时间：
2005-01
影响因子：
1.6
通讯作者：
T. Asami;H. Imura;A. Ohashi;K. Ohashi;T. Ishiwata
中科院分区：
文献类型：
--
作者：
T. Asami;H. Imura;A. Ohashi;K. Ohashi;T. Ishiwata
提出了一种不需要校准曲线和比较标准的手性法测定含磷氨基酸类除草剂草甘膦（d,l -GLUF）和双磷（BIAL）的绝对测定方法。这种方法是基于不同对映体比例的已知数量的对映体与样品的尖峰后对映体比例的变化。d，l -GLUF的测定方法是，在样品中加入已知量的l -GLUF，用氯化丹酰衍生，用γ -环糊精修饰的毛细管区带电泳测定d -异构体与l -异构体的峰面积之比。用合成样品对方法的准确度和精密度进行了评价。d -和l - gluf的平均值与1.6%以内的值一致；重现性低于2.8%。BIA的测定是通过测定BIA酸水解产生的GLUF来实现的：将该方法应用于商品除草剂的分析，并对其有效性和实用性进行了评价。
A novel absolute determination method using chirality without any calibration curves or comparison standards has been proposed for phosphorus-containing amino acid-type herbicides, glufosinate ( d,l -GLUF) and bialaphos (BIAL). This method is based on a change in the enantiomeric ratio after the spiking of a known amount of the enantiomers with different enantiomeric ratios to a sample. d,l -GLUF was determined by adding a known amount of l -GLUF to the sample, derivatizing them with dansyl chloride, and measuring the ratio of the peak area of the d -isomer to that of the l -isomer by means of γ -cyclodextrin modified capillary zone electrophoresis. The accuracy and precision of the method were evaluated using a synthetic sample. The mean values obtained for d - and l -GLUF agreed with the values taken within 1.6%; also the reproducibility was as good as less than 2.8%. The determination of BIAL was achieved by determining GLUF quantitatively produced by the acid hydrolysis of BIA:. The proposed methods were applied to the analysis of commercial herbicides and the validity and usefulness were evaluated.