THE MUTY GENE - A MUTATOR LOCUS IN ESCHERICHIA-COLI THAT GENERATES G.C-]T.A TRANSVERSIONS
THE MUTY GENE - A MUTATOR LOCUS IN ESCHERICHIA-COLI THAT GENERATES G.C-]T.A TRANSVERSIONS
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DOI：
10.1073/pnas.85.8.2709
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发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
MILLER, JH
中科院分区：
文献类型：
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作者：
NGHIEM, Y;CABRERA, M;MILLER, JH
我们已经使用了一个菌株与改变lacZ基因，它恢复到野生型，通过只有某些颠换，以检测在大肠杆菌中的颠换特异性突变。检测依赖于乳头状技术，该技术使用β-半乳糖苷显示蓝色Lac+乳头。一类突变子对G. cntdot是特异性的。C. T.cntdot.由一组lacZ突变的回复模式和lacI基因中正向无义突变的分布确定的颠换。负责增变基因表型的基因座被命名为mutY，并在E.杆菌mutY基因座可以以与先前表征的mutT基因座类似但相反的方式起作用，这导致A. cntdot。T.fwdarw. C. cntdot. G颠换。
We have used a strain with an altered lacZ gene, which reverts to wild type via only certain transversions to detect transversion-specific mutators in Escherichia coli. Detection relied on a papillation technique that uses a combination of .beta.-galactosides to reveal blue Lac+ papillae. One class of mutators is specific for the G .cntdot. C .fwdarw. T .cntdot. A transversion as determined by the reversion pattern of a set of lacZ mutations and by the distribution of forward nonsense mutations in the lacI gene. The locus responsible for the mutator phenotype is designated mutY and maps near 64 min on the genetic map of E. coli. The mutY locus may act in a similar but reciprocal fashion to the previously characterized mutT locus, which results in A .cntdot. T .fwdarw. C .cntdot. G transversions.