Human cytomegalovirus persistently infects aortic endothelial cells
Human cytomegalovirus persistently infects aortic endothelial cells
复制标题
DOI：
10.1128/jvi.72.7.5661-5668.1998
复制
发表时间：
1998-07-01
影响因子：
5.4
通讯作者：
Nelson, JA
中科院分区：
文献类型：
--
作者：
Fish, KN;Soderberg-Naucler, C;Nelson, JA
内皮细胞（EC）是人巨细胞病毒（HCMV）感染的重要细胞类型，微血管EC和大血管EC在不同器官表现出不同的生化和功能特性。微血管EC和大血管EC之间的表型差异可能改变这些细胞支持HCMV复制的能力。在本研究中，我们比较了HCMV在主动脉大血管EC（AEC）和脑微血管EC（BMVEC）中的复制。IE72，pp65，和gB病毒抗原表达BMVEC和AEC的免疫荧光检查显示感染细胞的频率相似。BMVEC细胞内的病毒产量比AEC高3个对数单位，而两种细胞类型中均产生等量的细胞外病毒。BMVEC的HCMV感染导致快速的细胞溶解，而病毒是非溶解性的，并且在培养物的寿命期间从HCMV感染的AEC中持续释放。电镜观察发现，在AEC和BMVEC中都有大量的核衣壳形成，而BMVEC的细胞质中有大量的成熟病毒颗粒。这些观察结果表明，HCMV在不同器官EC中的复制水平是不同的，并提示持续感染的AEC可能是病毒的储存库。
Endothelial cells (EC) have been implicated as constituting an important cell type in the pathogenesis of human cytomegalovirus (HCMV), Microvascular and macrovascular EC exhibit different biochemical and functional properties depending on the organ of origin. Phenotypic differences between microvascular and macrovascular EC may alter the ability of these cells to support HCMV replication, In this study, we compared the replication of HCMV in primary macrovascular aortic EC (AEC) with that in brain microvascular EC (BMVEC). An examination of IE72, pp65, and gB viral antigen expression in BMVEC and AEC by immuno-flourescence revealed similar frequencies of infected cells. Intracellular production of virus was 3 log units greater in BMVEC than in AEC, while equal quantities of extracellular virus were produced in both cell types. HCMV infection of BMVEC resulted in rapid cellular lysis, while the virus was nonlytic and continuously released from HCMV-infected AEC for the life span of the culture. An examination of infected cells by electron microscopy revealed the formation of abundant nucleocapsids in both AEC and BMVEC, However, significant amounts of mature viral particles were only detected in the cytoplasm of BMVEC, These observations indicate that levels of HCMV replication in EC obtained from different organs are distinct and suggest that persistently infected AEC may serve as a reservoir of virus.