IN SILICO ANALYSIS AND FUNCTIONAL CHARACTERIZATION OF FHUB, A COMPONENT OF ERWINIA MALLOTIVORA FERRIC HYDROXAMATE UPTAKE SYSTEM
IN SILICO ANALYSIS AND FUNCTIONAL CHARACTERIZATION OF FHUB, A COMPONENT OF ERWINIA MALLOTIVORA FERRIC HYDROXAMATE UPTAKE SYSTEM
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DOI：
10.11113/jt.v82.13635
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发表时间：
2020-05-01
期刊：
影响因子：
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通讯作者：
Abu Bakar, Norliza
中科院分区：
文献类型：
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作者：
Juri, Nor Mustaiqazah;Samsuddin, Aimera Farhana;Abu Bakar, Norliza
铁是细菌生长的关键元素，因为大多数致病细菌依赖于其宿主的铁供应。然而，铁源与宿主铁结合蛋白结合，并且需要特定的铁获取机制来螯合和运输铁到细菌。异羟肟酸铁摄取系统（Ferric hydroxamate uptake system，简称fhu）是一种将铁以异羟肟酸铁/铁色素的形式从细胞外环境输入细菌胞质溶胶的转运系统。本研究对食毛欧文氏菌（Erwinia mallotivora）fhu转运蛋白的一个重要组分fhuB进行了详细的电子结构分析。这为我们提供了蛋白质的结构特性，包括结构域和3D模型，系统发育分析和膜拓扑结构。为了进行功能分析，构建了fhuB基因敲除突变株，以评估在大肠杆菌中沉默该基因的效果。番木瓜的食毛孢侵染。与野生E.食草E.在引起番木瓜枯梢病症状时，食毛霉丧失了致病力。本研究结果揭示了铁的获得和代谢在大肠杆菌生长过程中的重要作用。食毛动物发病机制这突出了fhuB作为靶基因的作用和重要性; mallotivora为未来的研究和作为未来考虑的一部分，在番木瓜顶梢枯病管理策略。
Iron is a critical element for bacterial growth as most pathogenic bacteria relies on their host for iron supply. However, iron sources are bounded to the host iron binding protein and specific iron acquisition mechanism is required to chelate and transport the iron to the bacteria. Ferric hydroxamate uptake system or fhu is one of the transport systems that import iron in the form of ferric hydroxamate/ferrichrome from the extracellular environment into the bacterial cytosol. In this present study, a detailed in silico structural analysis was conducted on an important component of fhu transport member from Erwinia mallotivora named as fhuB. This provide us the structural properties of the protein which includes the domain and 3D model, phylogenetic analysis and the membrane topology. For functional analysis, a knockout mutant of fhuB gene strain was generated to evaluate the effect of silencing this gene during E. mallotivora infection in papaya. When compared to the wild E. mallotivora strain, fhuB mutant strain of E. mallotivora loss its virulence in causing dieback disease symptom in papaya. The result of this study has revealed the significant role of iron acquisition and metabolism during E. mallotivora pathogenesis. This highlights fhuB role and importance as the target gene; to inhibit iron uptake in E. mallotivora for future study and as a part of future consideration for dieback disease management strategy in papaya.