Two single descriptors of endothelial polymegethism and pleomorphism
Two single descriptors of endothelial polymegethism and pleomorphism
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DOI：
10.1007/s00417-010-1337-6
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Parafita, Manuel A.
中科院分区：
文献类型：
--
作者：
Gonzalez-Meijome, Jose Manuel;Jorge, Jorge;Parafita, Manuel A.
为了计算两个新的定量参数，直接反映水平的多形性和多形性使用的非接触式specular microscope提供的数据。我们检查了306眼（102男，204女），年龄范围从6至82岁（平均值+/- SD，44 +/- 22岁）。测定内皮细胞密度（ECD）、细胞平均大小（ACS）、细胞表面标准误（SEM）、细胞大小变异系数（CV）和六方指数（HI）。此外，还使用仪器分类获得的数据的加权线性组合推导出两个新指数：多形性（POLi）和多形性（PLEi），这些数据分别根据细胞大小和边数的个体计数对内皮细胞进行仪器分类，如仪器所提供的。糖尿病患者POLi和PLEi的平均值分别为10.47 ± 3.94和8.36 ± 1.21。POLi和PLEi与SEM显示高度相关性（r = 0.911，r = 0.664），ECD（r =-0.997，r = -0.585），ACS（r = 0.997，r = 0.441），ACS（SD）相关系数r = 0.883，r = 0.682，CV（r = 0.301，r = 0.712），HI（r =-0.437，r = -0.991）均为极显著相关（p < 0.001）。POLi和PLEi与年龄呈显著正相关（r = 0.765，p < 0.001和r = 0.428，p < 0.001）。POLi在一组糖尿病患者中显著高于另一组年龄匹配的对照组（p = 0.001）。两个单一的定量参数的内皮细胞多形性和多形性（POLi和PLEi分别）已从获得的数据与商业非接触式镜面反射显微镜。这些参数已被证明可以识别糖尿病患者和非糖尿病患者的角膜内皮之间的差异。
To compute two new quantitative parameters that directly reflect the level of polymegethism and pleomorphism using data provided by a non-contact specular microscope.We examined right eyes of 306 voluntaries (102 males, 204 females) whose ages ranged from 6 to 82 years (mean +/- SD, 44 +/- 22 years). Endothelial cell density (ECD), average cell size (ACS), standard error of cells surface (SEM), coefficient of variation in cell size (CV) and hexagonality index (HI) were obtained. In addition, two new indices of polymegethism (POLi) and pleomorphism (PLEi) were derived using weighted linear combinations of data obtained from instrument classification of endothelial cells based on the individual counts of cells by size and number of sides respectively, as provided by the instrument. Values of POLi and PLEi were compared between a group of diabetic patients and a group of age-matched controls.Average values of POLi and PLEi were 10.47 +/- 3.94 and 8.36 +/- 1.21 respectively. POLi and PLEi display high correlation with SEM (r = 0.911, r = 0.664), ECD (r = -0.997, r = -0.585), ACS (r = 0.997, r = 0.441), ACS (SD) (r = 0.883, r = 0.682), CV (r = 0.301, r = 0.712) and HI (r = -0.437, r = -0.991); all these correlations were highly significant (p < 0.001). POLi and PLEi also showed significant positive correlations with age (r = 0.765, p < 0.001 and r = 0.428, p < 0.001 respectively). POLi was significantly higher in a group of diabetic patients when compared with another group of age-matched controls (p = 0.001).Two single quantitative parameters of endothelial polymegethism and pleomorphism (POLi and PLEi respectively) have been derived from the data obtained with a commercial non-contact specular microscope. These parameters have been demonstrated to identify differences between the corneal endothelium of diabetic and non-diabetic patients.