Revealing low-dose radiation damage using single-crystal spectroscopy.
Revealing low-dose radiation damage using single-crystal spectroscopy.
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DOI：
10.1107/s0909049511004250
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发表时间：
2011-05
影响因子：
2.5
通讯作者：
Pearson AR
中科院分区：
文献类型：
--
作者：
Owen RL;Yorke BA;Gowdy JA;Pearson AR
在同步辐射源的X射线束的血红素蛋白质的快速减少的数据。使用单晶光谱检测这些变化和它们的含义从氧化物种的衍射数据收集进行了讨论。从X射线晶体学获得的结构信息和功能洞察力可以通过使用互补光谱来增强。在这里，可以从光谱方法结合X射线晶体学和最佳实践的单晶紫外-可见吸收数据收集通常使用的信息进行简要回顾。使用在瑞士光源的原位系统收集的数据，在100 K的低剂量X射线诱导的辐射损伤和金属蛋白中的溶剂化电子产生的时间和剂量尺度进行了研究。 本文还讨论了剂量率对这些尺度的影响。
Data on the rapid reduction of haem proteins in the X-ray beam at synchrotron sources are presented. The use of single-crystal spectroscopy to detect these changes and their implication for diffraction data collection from oxidized species is also discussed. The structural information and functional insight obtained from X-ray crystallography can be enhanced by the use of complementary spectroscopies. Here the information that can be obtained from spectroscopic methods commonly used in conjunction with X-ray crystallography and best-practice single-crystal UV-Vis absorption data collection are briefly reviewed. Using data collected with the in situ system at the Swiss Light Source, the time and dose scales of low-dose X-ray-induced radiation damage and solvated electron generation in metalloproteins at 100 K are investigated. The effect of dose rate on these scales is also discussed.