BIOLOGICALLY AND ACOUSTICALLY COMPATIBLE CHAMBER FOR STUDYING ULTRASOUND-MEDIATED DELIVERY OF THERAPEUTIC COMPOUNDS
BIOLOGICALLY AND ACOUSTICALLY COMPATIBLE CHAMBER FOR STUDYING ULTRASOUND-MEDIATED DELIVERY OF THERAPEUTIC COMPOUNDS
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DOI：
10.1016/j.ultrasmedbio.2015.03.020
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发表时间：
2015-07-01
影响因子：
2.9
通讯作者：
Stride, Eleanor
中科院分区：
文献类型：
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作者：
Carugo, Dario;Owen, Joshua;Stride, Eleanor
超声波（US）与微泡结合，已被发现是一种潜在的替代病毒疗法，用于去除生物细胞。然而，将这种技术转化到临床环境需要对实现期望的治疗效果所需的声学参数进行鲁棒的和系统的优化。目前，已经开发了各种不同的装置来体外检测细胞，导致缺乏标准化的实验条件，并且难以比较来自不同实验室的结果。为了克服这一局限性，我们提出了一种易于制造和成本效益的装置，用于US介导的治疗化合物的递送。它包括一个市售的细胞培养皿，与内部开发的硅基“盖”相结合，使器械能够浸入水浴中进行US暴露。这里描述的是该设备的设计，表征的声场和腔室内的流体动力学和治疗输送实验的示例协议。(C)2015年世界医学和生物学超声联合会。
Ultrasound (US), in combination with microbubbles, has been found to be a potential alternative to viral therapies for transfecting biological cells. The translation of this technique to the clinical environment, however, requires robust and systematic optimization of the acoustic parameters needed to achieve a desired therapeutic effect. Currently, a variety of different devices have been developed to transfect cells in vitro, resulting in a lack of standardized experimental conditions and difficulty in comparing results from different laboratories. To overcome this limitation, we propose an easy-to-fabricate and cost-effective device for application in US-mediated delivery of therapeutic compounds. It comprises a commercially available cell culture dish coupled with a silicon-based "lid'' developed in-house that enables the device to be immersed in a water bath for US exposure. Described here are the design of the device, characterization of the sound field and fluid dynamics inside the chamber and an example protocol for a therapeutic delivery experiment. (C) 2015 World Federation for Ultrasound in Medicine & Biology.