Super-highly hydroxylated fullerene derivative protects human keratinocytes from UV-induced cell injuries together with the decreases in intracellular ROS generation and DNA damages
Super-highly hydroxylated fullerene derivative protects human keratinocytes from UV-induced cell injuries together with the decreases in intracellular ROS generation and DNA damages
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DOI：
10.1016/j.jphotobiol.2010.09.006
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发表时间：
2011-01-10
影响因子：
5.4
通讯作者：
Miwa, Nobuhiko
中科院分区：
文献类型：
--
作者：
Saitoh, Yasukazu;Miyanishi, Akifumi;Miwa, Nobuhiko
多羟基富勒烯（富勒烯醇：C-60(OH)(n)）被认为是主要的水溶性富勒烯衍生物，其作为生物系统中的自由基清除剂或抗氧化剂具有特殊意义。最近，成功合成了新型多羟基富勒烯（C-60（OH）（44）中心点8H（2）O：SHH-F）。在本研究中，我们研究了三种富勒烯醇（C-60（OH）（6-12）：LH-F、C-60（OH）（32-34中心点）7H（2）O：HH-F和C-60（OH）（44）.8H（2）O：SHH-F）对紫外线照射引起的细胞损伤的自由基清除作用和细胞保护作用。 DMPO-自旋捕获/ESR方法显示，HH-F和SHH-F具有比LH-F更显着的羟基自由基清除活性。 HH-F 和 SHH-F 显着抑制 UVA 或 UVB 照射引起的人皮肤角质形成细胞 HaCaT 损伤，但 LF-H 几乎没有抑制作用。 SHH-F的细胞保护作用有优于HH-F的趋势。并且SHH-F对UVB引起的损伤的细胞保护作用比UVA更有效。 UVB 照射 HaCaT 细胞会导致细胞损伤相关症状迅速增加，例如细胞内氧化应激水平、环丁烷嘧啶二聚体的形成和染色质浓缩，所有这些都被 SHH-F 抑制。因此，SHH-F 可以预防 UVB 引起的多种有害影响，表明 SHH-F 通过清除角质形成细胞中的细胞内活性氧来发挥细胞保护作用。 (C) 2010 Elsevier B.V. 保留所有权利。
Polyhydroxylated fullerenes (fullerenols: C-60(OH)(n)) are known as the major water-soluble fullerene derivatives which possess particular significance as free radical scavengers or antioxidants in biological systems. Recently, the novel polyhydroxylated fullerene (C-60 (OH)(44)center dot 8H(2)O: SHH-F) was successfully synthesized. In the present study, we investigated the radical-scavenging effects and cytoprotective effects of three types of fullerenols (C-60(OH)(6-12): LH-F, C-60 (OH)(32-34 center dot)7H(2)O: HH-F, and C-60 (OH)(44).8H(2)O: SHH-F) on UV-irradiation-induced cell injuries. HH-F and SHH-F exerted hydroxyl-radical scavenging activities as shown by DMPO-spin trap/ESR method, more markedly than LH-F. UVA or UVB irradiation-induced injuries in human skin keratinocytes HaCaT were significantly suppressed by HH-F and SHH-F, but scarcely by LF-H. The cytoprotective effects of SHH-F had a tendency to be superior to that of HH-F. And the cytoprotective effects of SHH-F against UVB-induced injuries were more effective than those of UVA. Irradiation with UVB to HaCaT cells was shown to cause rapid increases in cell-injury-associated symptoms such as intracellular oxidative stress levels, the formation of cyclobutane pyrimidine dimers and chromatin condensation, all of which were repressed by SHH-F. Thus, UVB-induced diverse harmful effects could be prevented by SHH-F, which was suggested to exert the cytoprotective effects through intracellular reactive oxygen species-scavenging in the keratinocytes. (C) 2010 Elsevier B.V. All rights reserved.