Expression of SOD gene and evaluating its role in stress tolerance in NaCl and PEG stressed Lycopersicum esculentum
Expression of SOD gene and evaluating its role in stress tolerance in NaCl and PEG stressed Lycopersicum esculentum
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DOI：
10.3906/bot-1305-1
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发表时间：
2014-01-01
影响因子：
1.8
通讯作者：
Aras, Emine Sumer
中科院分区：
文献类型：
--
作者：
Aydin, Semra;Buyuk, Ilker;Aras, Emine Sumer
抗氧化酶机制对于保护作物生产力免受胁迫环境条件的影响是重要的。因此，本研究旨在评估番茄（Lycopersicum esculentum L.）的脂质过氧化（通过丙二醛（MDA））水平，超氧化物歧化酶（SOD）基因表达谱和SOD酶活性。植物经受不同浓度（100和150 mM）和时间段的NaCl和聚乙二醇（PEG）胁迫（3 h、6 h、9 h、12 h和48 h）。植物体内脂质过氧化作用的增强可能是由于活性氧（ROS）积累增加所致。结果表明，番茄植株的抗氧化反应可以通过SOD基因转录水平和酶活性的变化来反映，但SOD基因表达谱与SOD酶活性变化之间没有明显的正相关性。结果，胁迫条件导致番茄植物的胁迫（由MDA水平增加指示）并触发SOD基因的表达水平。番茄植株对PEG胁迫的抗氧化反应比对NaCl胁迫的反应更强烈。这些结果表明SOD在番茄抗逆中的重要性。SOD基因在NaCl和PEG胁迫下的过表达和沉默仍有待于进一步的研究。
Antioxidant enzyme mechanisms are important for protecting crop productivity against stressful environmental conditions. Therefore, the current study was designed to evaluate lipid peroxidation (via malondialdehyde (MDA)) levels, superoxide dismutase (SOD) gene expression profiles, and SOD enzyme activities in tomato (Lycopersicum esculentum L.) plants subjected to different concentrations (100 and 150 mM) and time periods of NaCl and polyethylene glycol (PEG) stress (3 h, 6 h, 9 h, 12 h, and 48 h). Enhancement of lipid peroxidation in plants may be attributed to the increased accumulation of reactive oxygen species (ROS). Results indicated the antioxidant responses of tomato plants could be reflected as changes in gene transcripts and enzyme activities of SOD, but SOD gene expression patterns and changes in SOD enzyme activity revealed no positive correlation. As a result, stressful conditions led to stress in tomato plants (indicated by increased MDA levels) and triggered the expression levels of the SOD gene. The antioxidative response of tomato plants to PEG stress was found to be more vigorous than the response to NaCl stress. All these results pointed to the importance of SOD for stress defense in tomato. The overexpression and silencing of the SOD gene in tomato plants under NaCl and PEG stresses remain to be identified in further studies.