MUTAGENICITY AND CLASTOGENICITY OF 3-CHLORO-4-(DICHLOROMETHYL)-5-HYDROXY-2(5H)-FURANONE (MX) IN L5178Y/TK+/--3.7.2C MOUSE LYMPHOMA-CELLS
MUTAGENICITY AND CLASTOGENICITY OF 3-CHLORO-4-(DICHLOROMETHYL)-5-HYDROXY-2(5H)-FURANONE (MX) IN L5178Y/TK+/--3.7.2C MOUSE LYMPHOMA-CELLS
复制标题
DOI：
10.1016/0165-7992(95)00052-6
复制
发表时间：
1995-11-01
期刊：
影响因子：
--
通讯作者：
MOORE, MM
中科院分区：
文献类型：
--
作者：
HARRINGTONBROCK, K;DOERR, CL;MOORE, MM
3-氯-4-（二氯甲基）-5-羟基-2(5H)-呋喃酮（MX）在L5178Y/TK+/—3.7.2C小鼠淋巴瘤细胞中不经外源激活检测胸苷激酶位点突变和致裂性。在0.75 μ g/ml浓度下，诱导突变频率为1027 / 10(6)例（存活率为11%）。观察到大小菌落突变体的浓度相关增加，但大多数MX诱导突变体形成小菌落，与观察到的阳性破胚反应一致。在0.75 μ g/ml剂量下，MX主要诱导染色单体断裂和重排（每100个细胞有30个染色单体和4个染色体畸变）。这些研究表明MX诱导广泛的遗传损伤。
3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) was tested without exogenous activation in L5178Y/TK+/--3.7.2C mouse lymphoma cells for mutation at the thymidine kinase locus and for clastogenicity. At a concentration of 0.75 mu g/ml, the induced mutant frequency was 1027 per 10(6) survivors (survival=11%). A concentration-related increase of large and small colony mutants was observed, but the majority of the MX induced mutants formed small colonies, consistent with the positive clastogenic response that was observed. MX primarily induced chromatid breaks and rearrangements (30 chromatid and 4 chromosome aberrations per 100 cells) at the 0.75 mu g/ml dose. These studies indicate that MX induces a broad spectrum of genetic damage.