Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes.
Coding sequence and expression patterns of mouse chordin and mapping of the cognate mouse chrd and human CHRD genes.
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DOI：
10.1006/geno.1998.5474
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发表时间：
1998-09
期刊：
影响因子：
4.4
通讯作者：
W. Pappano;Ian C. Scott;Timothy G. Clark;Roger L. Eddy;T. B. Shows;D. Greenspan
中科院分区：
文献类型：
--
作者：
W. Pappano;Ian C. Scott;Timothy G. Clark;Roger L. Eddy;T. B. Shows;D. Greenspan
脊索蛋白是一种关键的发育蛋白，其通过结合腹侧化TGF-β样骨形态发生蛋白并将它们隔离在潜在复合物中来背侧化早期脊椎动物胚胎组织。在这里，我们报告的第一个哺乳动物脊索蛋白的特性。小鼠脊索蛋白的全长cDNA序列，RNA印迹分析表明，小鼠脊索蛋白基因Chrd表达在相对较高的水平在7天postcoplant小鼠胚胎和在以后的发展时间和成人组织中的水平大大降低。这些结果意味着脊索蛋白在哺乳动物胚胎的原肠胚形成过程中的重要作用。然而，鼠和人的脊索蛋白基因都显示在几个胎儿和成人组织中以容易检测的水平表达，最明显的是肝脏和小脑，这表明在器官发生和体内平衡中的额外作用。通过种间杂交将CHRD定位于小鼠16号染色体上，通过辐射杂交定位将同源人类基因CHRD定位于人类3q 27。
Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. Here we report the first characterization of mammalian chordin. The full-length cDNA sequence for mouse chordin is given, and RNA blot analysis shows the murine chordin gene Chrd to be expressed at relatively high levels in 7-day postcoitum mouse embryos and at much decreased levels at later developmental times and in adult tissues. These results imply a major role for chordin during gastrulation of the mammalian embryo. Nevertheless, both murine and human chordin genes are shown to be expressed at readily detectable levels in several fetal and adult tissues, most notably liver and cerebellum, suggesting additional roles in organogenesis and homeostasis. Chrd was mapped to mouse chromosome 16 using interspecific crosses, and the cognate human gene CHRD was localized to human chromosome 3q27 by radiation hybrid mapping.