Glutamine is required for snakehead fish vesiculovirus propagation via replenishing the tricarboxylic acid cycle
Glutamine is required for snakehead fish vesiculovirus propagation via replenishing the tricarboxylic acid cycle
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黑鱼水泡病毒通过补充三羧酸循环繁殖需要谷氨酰胺
DOI：
10.1099/jgv.0.000597
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发表时间：
2016
影响因子：
3.8
通讯作者：
Lin Li
中科院分区：
文献类型：
--
作者：
Sun Lindan;Yi Lizhu;Zhang Chi;Liu Xiaodan;Feng Shuangshuang;Chen Wenjie;Lan Jiangfeng;Zhao Lijuan;Tu Jiagang;Lin Li
蛇头鱼水泡病毒（SHVV）是弹状病毒科的一员，在我国蛇头鱼养殖业中已引起大量死亡。以往对SHVV感染和未感染的条纹蛇头鱼细胞（SSN-1）的转录组测序表明，谷氨酰胺酶(GLS) 是谷氨酰胺代谢的关键酶，在 SHV 感染时上调。因此，我们更加关注谷氨酰胺在 SHV 传播中的作用。谷氨酰胺剥夺显着降低了SHVV的mRNA和蛋白的表达以及病毒颗粒的产生，表明SHVV的繁殖需要谷氨酰胺。谷氨酰胺可以通过GLS转化为谷氨酸，然后转化为酮戊二酸，添加TCA循环中间体茶酮戊二酸、草酰乙酸或丙酮酸显着恢复SHVV传播，表明SHVV传播需要谷氨酰胺是由于其补充通过抑制剂双-2-(5-苯基乙酰氨基-1,3,4-噻二唑-2-基)乙基硫醚抑制SSN-1细胞中GLS的活性，降低。SHVV传播，而过表达GLS 增加了 SHVV 传播。总之，我们的数据揭示了谷氨酰胺代谢与 SHVV 传播之间的关系。
Snakeheadfishvesiculovirus(SHVV),amemberofthefamilyRhabdoviridae,hascausedmass.mortalityinsnakeheadfishcultureinChina.PrevioustranscriptomicsequencingofSHVV-infected.andnon-infectedstripedsnakeheadfishcells(SSN-1)showedthatglutaminase (GLS),thecritical.enzymeofglutaminemetabolism,wasupregulateduponSHVVinfection.Itthereforedrewour.attentiontoinvestigatingtheroleofglutamineinSHVVpropagation.Glutaminedeprivation.significantlyreducedtheexpressionofthemRNAsandproteinsofSHVV,andtheproductionof.virusparticles,indicatingthatglutaminewasrequiredforSHVVpropagation.Glutaminecanbe.convertedtoglutamatebyGLS,andthenbeconvertedtoa-ketoglutarate, tojoininthetricarboxylic.acid(TCA)cycle.AdditionoftheTCAcycleintermediatea-ketoglutarate,oxaloaceticacidor.pyruvatesignificantlyrestoredSHVVpropagation,indicatingthattherequirement ofglutaminefor.SHVVpropagationwasduetoitsreplenishment oftheTCAcycle.InhibitingtheactivityofGLSin.SSN-1cellsbyaninhibitor,bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide,decreased.SHVVpropagation,whileoverexpression ofGLSincreasedSHVVpropagation.Takentogether,our.datahaverevealedtherelationshipbetweenglutaminemetabolismandSHVVpropagation.