Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice
Neo-functionalization of a Teosinte branched 1 homologue mediates adaptations of upland rice
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Teosinte Branched 1 同源物的新功能化介导旱稻的适应
DOI：
10.1038/s41467-019-14264-1
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发表时间：
2020-02-05
影响因子：
16.6
通讯作者：
Hu, Fengyi
中科院分区：
文献类型：
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作者：
Lyu, Jun;Huang, Liyu;Hu, Fengyi
玉米驯化基因Teosinte branched 1（Tb1）的水稻直向同源基因影响分蘖。但是，与玉米Tb1基因不同，它在驯化过程中没有被选择。在此，我们报告了一个OsTb1重复基因（OsTb2）已被人工选择在高地适应和OsTb2的自然变异与分蘖数。有趣的是，过量表达该基因的转基因水稻表现出增加而不是减少分蘖，这表明OsTb2获得了与OsTb1相反的调节作用。功能分析表明，OsTb2蛋白通过与同源OsTb1蛋白相互作用，对水稻分蘖起正向调节作用，并抵消OsTb1对水稻分蘖的抑制作用。我们进一步表征了OsTb2内分别调节蛋白质功能和基因表达的两个功能变异。这些结果不仅提供了一个新功能化的例子，产生一个相反的功能后，重复，但也表明，theB1同源物已被选定在高地。
The rice orthologue of maize domestication geneTeosinte branched 1(Tb1) affects tillering. But, unlike maizeTb1gene, it was not selected during domestication. Here, we report that anOsTb1duplicate gene (OsTb2) has been artificially selected during upland rice adaptation and that natural variation inOsTb2is associated with tiller number. Interestingly, transgenic rice overexpressing this gene shows increased rather than decreased tillering, suggesting thatOsTb2gains a regulatory effect opposite to that ofOsTb1following duplication. Functional analyses suggest that the OsTb2 protein positively regulates tillering by interacting with the homologous OsTb1 protein and counteracts the inhibitory effect of OsTb1 on tillering. We further characterize two functional variations withinOsTb2that regulate protein function and gene expression, respectively. These results not only present an example of neo-functionalization that generates an opposite function following duplication but also suggest that theTb1homologue has been selected in upland rice.