miR-200b mediates post-transcriptional repression of ZFHX1B
miR-200b mediates post-transcriptional repression of ZFHX1B
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DOI：
10.1261/rna.586807
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发表时间：
2007-08-01
期刊：
影响因子：
4.5
通讯作者：
Lund, Anders H.
中科院分区：
文献类型：
--
作者：
Christoffersen, Nanna Ronbjerg;Silahtaroglu, Asli;Lund, Anders H.
MicroRNA 通过对其同源 mRNA 靶标进行转录后调节，在动物发育和体内平衡过程中发挥重要作用。 ZFHX1B 是一种转录抑制因子，参与 TGF beta 信号通路以及通过 E-钙粘蛋白的调节参与上皮细胞到间质细胞的转变过程。我们表明，Zfhx1b 和 miR-200b 在成年小鼠大脑中局部共表达，并且 miR-200b 通过 3'-非翻译区中存在的多个序列元件抑制 Zfhx1b 的表达。 miR-200b 的过度表达会导致内源性 ZFHX1B 的抑制，而抑制 miR-200b 则会缓解 ZFHX1B 的抑制。根据这些发现，miR-200b 调节 E-钙粘蛋白启动子的活性。
MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGF beta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'- untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.