Genome characterization of a GII.6 norovirus strain identified in China.
Genome characterization of a GII.6 norovirus strain identified in China.
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DOI：
10.1016/j.meegid.2015.01.027
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发表时间：
2015-04
期刊：
影响因子：
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通讯作者：
L. Xue;Qingping Wu;X. Kou;W. Cai;Jumei Zhang;Weipeng Guo
中科院分区：
文献类型：
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作者：
L. Xue;Qingping Wu;X. Kou;W. Cai;Jumei Zhang;Weipeng Guo
诺如病毒（NoVs）是世界范围内非细菌性急性胃肠炎的主要病因。大多数NoV感染是由GII.4引起的，但GII.6也是一个重要的基因型，在人群中长期存在。本研究对中国分离的NoV菌株GZ2010-L96的全基因组序列进行了鉴定和系统发育分析。该病毒基因组包含7550个核苷酸，系统发育分析显示该病毒属于GII.6基因型。收集所有已报道的GII.6 NoV衣壳蛋白序列进行比较分析，将GZ2010-L96与其他8株聚类为GII.6-b。同时，经多重比对发现，病毒衣壳上53个位点具有亚簇特异性。此外，GZ2010-L96与GII.4 VA387株的同源建模显示出不同的抗原分布模式。综上所述，在中国检测到的GII.6菌株GZ2010-L96的基因组已被广泛表征，基于衣壳蛋白的GII.6 NoVs系统发育分析可能揭示了与优势基因型GII.4不同的进化过程。
Noroviruses (NoVs) are the primary cause of non-bacterial acute gastroenteritis worldwide. Most NoV infections are caused by GII.4, but GII.6 is also an important genotype with a long-term persistence in human populations. In this study, the complete genome sequence of a NoV strain GZ2010-L96 isolated in China was identified and analyzed phylogenetically. The viral genome comprised 7550 nucleotides, and its phylogenetic analysis revealed that the strain belonged to GII.6 genotype. All reported GII.6 NoV capsid protein sequences were also collected for comparative analysis, and GZ2010-L96 was clustered into GII.6-b with other 8 strains. Meanwhile, it was found that 53 spots on viral capsid showed subcluster specificity according to multiple alignments. Moreover, homologous modeling of GZ2010-L96 based on comparison with GII.4 VA387 strain showed a different antigen distribution pattern. In summary, the genome of the GII.6 strain GZ2010-L96 detected in China was extensively characterized, and phylogenetic analyses of GII.6 NoVs based on the capsid proteins may reveal a different evolution process from the predominant genotype GII.4.