NATURE OF INHIBITOR OF CELL-FREE PROTEIN-SYNTHESIS FORMED IN RESPONSE TO INTERFERON AND DOUBLE-STRANDED-RNA
NATURE OF INHIBITOR OF CELL-FREE PROTEIN-SYNTHESIS FORMED IN RESPONSE TO INTERFERON AND DOUBLE-STRANDED-RNA
复制标题
DOI：
10.1038/268540a0
复制
发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
HOVANESSIAN, AG
中科院分区：
文献类型：
--
作者：
KERR, IM;BROWN, RE;HOVANESSIAN, AG
用抗病毒剂干扰素 1 预处理的小鼠 L 细胞的无细胞系统中的蛋白质合成显示出对双链 RNA (dsRNA) 抑制的敏感性增强（参考文献 2-4）。一种 dsRNA 依赖性蛋白激酶 5-7 和一种热稳定的低分子量蛋白质合成抑制剂（LMW 抑制剂）似乎可能与 ATP 和 dsRNA 孵育时在此类系统中形成（参考文献 7）有关。我们已经证明，LMW 抑制剂可以使用高度纯化的酶级分方便地合成，该酶级分来自干扰素处理的细胞，与惰性支持物上的 dsRNA 结合。在这里，我们报告其部分特征。
PROTEIN synthesis in cell-free systems from mouse L-cells pretreated with the antiviral agent interferon1shows an enhanced sensitivity to inhibition by double-stranded RNA (dsRNA) (refs 2–4). A dsRNA-dependent protein kinase5–7and a heat-stable, low-molecular-weight inhibitor (LMW inhibitor) of protein synthesis which is formed in such systems on incubation with ATP and dsRNA (ref. 7), seem likely to be involved. We have shown8that the LMW inhibitor can be conveniently synthesised using a highly-purified enzyme fraction from interferon-treated cells bound to dsRNA on an inert support. Here we report its partial characterisation.