NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar).
NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar).
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DOI：
10.1016/j.margen.2016.06.004
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发表时间：
2017-02
期刊：
影响因子：
1.9
通讯作者：
Hazlerigg DG
中科院分区：
文献类型：
--
作者：
Lorgen M;Jorgensen EH;Jordan WC;Martin SA;Hazlerigg DG
溯河产卵的大西洋鲑鱼利用淡水和盐水（FW和SW）的栖息地在其生命周期。幼鱼转化是幼鱼从FW适应幼鱼向SW适应幼鱼发育的一个重要转变。在PST期间，调节组织，特别是鳃的生理变化是维持有效离子调节的关键。变化开始前SW曝光（准备阶段），并完成时，小鲑鱼进入海（激活阶段），渗透压可能会直接刺激基因表达的变化。在本文中，我们确定了4个核因子的活化T细胞（NFAT 5，渗透压应激转录因子）的旁系同源物在大西洋鲑鱼，这表明了很强的同源性，其特征功能域与其他脊椎动物中发现的。两个确定的旁系同源物（NFAT5B1和NFAT5B2）表现出增加的表达后，从FW转移到SW。这种效果是最大的parr，在短日照下维持，并显示最高的增加氯离子水平在响应SW曝光。这项研究的结果表明，NFAT 5参与了大西洋鲑鱼的渗透胁迫反应。
The anadromous Atlantic salmon utilizes both fresh and salt water (FW and SW) habitats during its life cycle. The parr-smolt transformation (PST) is an important developmental transition from a FW adapted juvenile parr to a SW adapted smolt. Physiological changes in osmoregulatory tissues, particularly the gill, are key in maintaining effective ion regulation during PST. Changes are initiated prior to SW exposure (preparative phase), and are completed when smolts enter the sea (activational phase) where osmotic stress may directly stimulate changes in gene expression. In this paper we identify 4 nuclear factor of activated T cells (NFAT5, an osmotic stress transcription factor) paralogues in Atlantic salmon, which showed strong homology in characterized functional domains with those identified in other vertebrates. Two of the identified paralogues (NFAT5b1 and NFAT5b2) showed increased expression following transfer from FW to SW. This effect was largest in parr that were maintained under short day photoperiod, and showed the highest increases in chloride ion levels in response to SW exposure. The results of this study suggest that NFAT5 is involved in the osmotic stress response of Atlantic salmon.