The HECATE genes regulate female reproductive tract development in Arabidopsis thaliana
The HECATE genes regulate female reproductive tract development in Arabidopsis thaliana
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DOI：
10.1242/dev.011510
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发表时间：
2007-10-15
期刊：
影响因子：
4.6
通讯作者：
Yanofsky, Martin F.
中科院分区：
文献类型：
--
作者：
Gremski, Kristina;Ditta, Gary;Yanofsky, Martin F.
在植物中成功受精需要雌性生殖组织的适当协调发展，包括柱头、花柱、隔膜和传递道。我们已经鉴定出三个密切相关的基因，HECATE1(HEC1)，HECATE2(HEC2)和HECATE3(HEC3)，它们的表达结构域围绕着拟南芥雌蕊的这些区域。HEC基因编码具有重叠功能的碱性螺旋-环-螺旋(BHLH)转录因子。根据HEC功能缺失的程度，植物表现出不同程度的不育性，隔膜、传输道和柱头发育缺陷，以及花粉管生长受损。观察到的表型与已报道的spatula(Spt)基因突变相似，spt基因也编码同一女性组织发育所需的bHLH转录因子。我们发现HEC蛋白可以在酵母双杂交系统中与SPT二聚化，这表明HEC基因与SPT协同工作，协调调节雌性生殖道的发育。此外，当HEC基因从CaMV 35S启动子异位表达时，一些转基因植株显示出针状的花序，这表明HEC基因可能参与了生长素介导的雌蕊群模式的控制。
Successful fertilization in plants requires the properly coordinated development of female reproductive tissues, including stigma, style, septum and transmitting tract. We have identified three closely related genes, HECATE1 ( HEC1), HECATE2 ( HEC2) and HECATE3 ( HEC3), the expression domains of which encompass these regions of the Arabidopsis gynoecium. The HEC genes encode putative basic helix- loop- helix ( bHLH) transcription factors with overlapping functionality. Depending on the amount of HEC function missing, plants exhibit varying degrees of infertility, defects in septum, transmitting tract and stigma development and impaired pollen tube growth. The observed phenotypes are similar to those reported for mutations in the SPATULA ( SPT) gene, which also encodes a bHLH transcription factor required for development of the same female tissues. We show that the HEC proteins can dimerize with SPT in a yeast two- hybrid system, indicating that the HEC genes work in concert with SPT to coordinately regulate development of the female reproductive tract. Furthermore, when the HEC genes are ectopically expressed from the CaMV 35S promoter, some of the resulting transgenic plants show pin- shaped inflorescences, suggesting that the HEC genes are probably involved in auxin- mediated control of gynoecium patterning.