The Class III Peroxidase (POD) Gene Family in Cassava: Identification, Phylogeny, Duplication, and Expression
The Class III Peroxidase (POD) Gene Family in Cassava: Identification, Phylogeny, Duplication, and Expression
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木薯中的 III 类过氧化物酶 (POD) 基因家族：鉴定、系统发育、复制和表达
DOI：
10.3390/ijms20112730
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发表时间：
2019-06-01
影响因子：
5.6
通讯作者：
Hu, Wei
中科院分区：
文献类型：
--
作者：
Wu, Chunlai;Ding, Xupo;Hu, Wei
III类过氧化物酶（POD）参与植物发育、激素信号传导和胁迫反应。然而，人们对木薯中的POD家族知之甚少。本研究共鉴定出91个木薯POD基因（mepod），并通过系统发育分析将其分为6个亚群。保守基序分析表明MePOD蛋白具有典型的过氧化物酶结构域，基因结构分析表明MePOD基因具有1 ~ 9个外显子。重复模式分析表明串联重复在MePOD基因扩增中发挥了作用。综合转录组学分析表明，MePOD基因参与了木薯的干旱响应和采后生理退化。在施加各种胁迫和相关信号处理后，几种mepod发生了转录变化。总之，我们对木薯POD家族进行了鉴定，揭示了POD基因在不同胁迫和采后生理劣化条件下的转录调控。这些结果可用于鉴定提高木薯作物抗逆性的潜在靶基因。
The class III peroxidase (POD) enzymes participate in plant development, hormone signaling, and stress responses. However, little is known about the POD family in cassava. Here, we identified 91 cassava POD genes (MePODs) and classified them into six subgroups using phylogenetic analysis. Conserved motif analysis demonstrated that all MePOD proteins have typical peroxidase domains, and gene structure analysis showed that MePOD genes have between one and nine exons. Duplication pattern analysis suggests that tandem duplication has played a role in MePOD gene expansion. Comprehensive transcriptomic analysis revealed that MePOD genes in cassava are involved in the drought response and postharvest physiological deterioration. Several MePODs underwent transcriptional changes after various stresses and related signaling treatments were applied. In sum, we characterized the POD family in cassava and uncovered the transcriptional control of POD genes in response to various stresses and postharvest physiological deterioration conditions. These results can be used to identify potential target genes for improving the stress tolerance of cassava crops.